Sökning: onr:"swepub:oai:DiVA.org:su-212292" > Spatial genomics ma...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 04666naa a2200613 4500 | |
001 | oai:DiVA.org:su-212292 | |
003 | SwePub | |
008 | 221206s2022 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:uu-492447 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-2122922 URI |
024 | 7 | a https://doi.org/10.1038/s41586-022-05425-22 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4924472 URI |
040 | a (SwePub)sud (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Lomakin, Artem4 aut |
245 | 1 0 | a Spatial genomics maps the structure, nature and evolution of cancer clones |
264 | c 2022-11-09 | |
264 | 1 | b Springer Science and Business Media LLC,c 2022 |
338 | a print2 rdacarrier | |
520 | a Genome sequencing of cancers often reveals mosaics of different subclones present in the same tumour. Although these are believed to arise according to the principles of somatic evolution, the exact spatial growth patterns and underlying mechanisms remain elusive. Here, to address this need, we developed a workflow that generates detailed quantitative maps of genetic subclone composition across whole-tumour sections. These provide the basis for studying clonal growth patterns, and the histological characteristics, microanatomy and microenvironmental composition of each clone. The approach rests on whole-genome sequencing, followed by highly multiplexed base-specific in situ sequencing, single-cell resolved transcriptomics and dedicated algorithms to link these layers. Applying the base-specific in situ sequencing workflow to eight tissue sections from two multifocal primary breast cancers revealed intricate subclonal growth patterns that were validated by microdissection. In a case of ductal carcinoma in situ, polyclonal neoplastic expansions occurred at the macroscopic scale but segregated within microanatomical structures. Across the stages of ductal carcinoma in situ, invasive cancer and lymph node metastasis, subclone territories are shown to exhibit distinct transcriptional and histological features and cellular microenvironments. These results provide examples of the benefits afforded by spatial genomics for deciphering the mechanisms underlying cancer evolution and microenvironmental ecology. | |
650 | 7 | a NATURVETENSKAPx Biologix Genetik0 (SwePub)106092 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Genetics0 (SwePub)106092 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
700 | 1 | a Svedlund, Jessicau Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)4 aut0 (Swepub:su)jesv4890 |
700 | 1 | a Strell, Carinau Uppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Uppsala University, Sweden,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden4 aut0 (Swepub:uu)carst810 |
700 | 1 | a Gataric, Milana4 aut |
700 | 1 | a Shmatko, Artem4 aut |
700 | 1 | a Rukhovich, Gleb4 aut |
700 | 1 | a Park, Jun Sung4 aut |
700 | 1 | a Ju, Young Seok4 aut |
700 | 1 | a Dentro, Stefan4 aut |
700 | 1 | a Kleshchevnikov, Vitalii4 aut |
700 | 1 | a Vaskivskyi, Vasyl4 aut |
700 | 1 | a Li, Tong4 aut |
700 | 1 | a Bayraktar, Omer Ali4 aut |
700 | 1 | a Pinder, Sarah4 aut |
700 | 1 | a Richardson, Andrea L.4 aut |
700 | 1 | a Santagata, Sandro4 aut |
700 | 1 | a Campbell, Peter J.4 aut |
700 | 1 | a Russnes, Hege4 aut |
700 | 1 | a Gerstung, Moritz4 aut |
700 | 1 | a Nilsson, Matsu Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)4 aut0 (Swepub:su)matsn |
700 | 1 | a Yates, Lucy R.4 aut |
710 | 2 | a Stockholms universitetb Institutionen för biokemi och biofysik4 org |
773 | 0 | t Natured : Springer Science and Business Media LLCg 611:7936, s. 594-602q 611:7936<594-602x 0028-0836x 1476-4687 |
856 | 4 | u https://doi.org/10.1038/s41586-022-05425-2y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1724036/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-212292 |
856 | 4 8 | u https://doi.org/10.1038/s41586-022-05425-2 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-492447 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
Kopiera och spara länken för att återkomma till aktuell vy