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The prodomain of Ss...
The prodomain of Ssy5 protease controls receptor-activated proteolysis of transcription factor Stp1
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- Pfirrmann, Thorsten (författare)
- Stockholms universitet,Avdelningen för cellbiologi
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- Heessen, Stijn (författare)
- Stockholms universitet,Avdelningen för cellbiologi
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- Omnus, Deike J., 1981- (författare)
- Stockholms universitet,Avdelningen för cellbiologi
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- Andréasson, Claes (författare)
- Stockholms universitet,Avdelningen för cellbiologi
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- Ljungdahl, Per O. (författare)
- Stockholms universitet,Avdelningen för cellbiologi
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(creator_code:org_t)
- 2010
- 2010
- Engelska.
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Ingår i: Molecular and Cellular Biology. - 0270-7306 .- 1098-5549. ; 30:13, s. 3299-309
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Extracellular amino acids induce the yeast SPS sensor to endoproteolytically cleave transcription factors Stp1 and Stp2 in a process termed receptor-activated proteolysis (RAP). Ssy5, the activating endoprotease, is synthesized with a large N-terminal prodomain and a C-terminal chymotrypsin-like catalytic (Cat) domain. During biogenesis, Ssy5 cleaves itself and the prodomain and Cat domain remain associated, forming an inactive primed protease. Here we show that the prodomain is a potent inhibitor of Cat domain activity and that its inactivation is a requisite for RAP. Accordingly, amino acid-induced signals trigger proteasome-dependent degradation of the prodomain. A mutation that stabilizes the prodomain prevents Stp1 processing, whereas destabilizing mutations lead to constitutive RAP-independent Stp1 processing. We fused a conditional degron to the prodomain to synthetically reprogram the amino acid-responsive SPS signaling pathway, placing it under temperature control. Our results define a regulatory mechanism that is novel for eukaryotic proteases functioning within cells
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Cell and molecular biology
- Cell- och molekylärbiologi
- Cell Biology
- cellbiologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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