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GVHD after chemothe...
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Sadeghi, B.Karolinska Institutet
(författare)
GVHD after chemotherapy conditioning in allogeneic transplanted mice
- Artikel/kapitelEngelska2008
Förlag, utgivningsår, omfång ...
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2008-09-29
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Springer Science and Business Media LLC,2008
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-57718
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-57718URI
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https://doi.org/10.1038/bmt.2008.261DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:117985643URI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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authorCount :7
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GVHD is a major complication in allogeneic SCT. Available GVHD models are mainly based on radio-therapy-conditioning and/or immune deficient mice. GVHD models based on chemotherapy-based regimens remain poorly studied, despite 50% of all transplantations being chemotherapy based. Our aim was to develop a GVHD model using chemotherapy as conditioning. Female BALB/c (H-2Kd) were conditioned with BU-CY and transplanted with 2 x 10(7) BM and 3 x 10(7) spleen cells from either C57BL/6 (H-2Kb) mice ( allogeneic setting) or from male BALB/c to serve as a control group for regimen-related toxicity and engraftment. GVHD manifestations and histopathological changes were evaluated. Chimerism and donor T cells presence in skin, intestine and liver were studied using FACS-, FISH analysis and immunohistochemistry. Allogeneic transplanted mice developed lethal GVHD starting from day+7 with both histological and clinical signs. Donor T cells accumulated in recipient skin and intestine with GVHD progression. BM-failure, apoptosis and T-lymphocyte infiltration into target organs were significantly higher in allogeneic when compared with the syngeneic group. No toxicity or GVHD signs were observed in the syngeneic setting. We report a mouse model of GVHD using BU-CY conditioning that represents the most common myeloablative-conditioning regimen in clinical SCT. This model can be utilized to study the role of conditioning on mechanisms underlying GVHD.
Ämnesord och genrebeteckningar
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BU
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CY
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chemotherapy conditioning
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GVHD
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mouse model
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hematopoietic stem cell transplantation
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versus-host-disease
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bone-marrow-transplantation
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total-body irradiation
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minor histocompatibility antigens
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stem-cell transplantation
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acute myeloid-leukemia
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t-cells
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in-vivo
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randomized-trial
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lethal graft
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NATURAL SCIENCES
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NATURVETENSKAP
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Aghdami, N.
(författare)
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Hassan, Z.Karolinska Institutet
(författare)
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Forouzanfar, M.
(författare)
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Rozell, B.Karolinska Institutet
(författare)
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Abedi-Valugerdi, ManuchehrKarolinska Institutet,Stockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)mabed
(författare)
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Hassan, M.Karolinska Institutet
(författare)
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Karolinska InstitutetInstitutionen för biokemi och biofysik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Bone Marrow Transplantation: Springer Science and Business Media LLC42:12, s. 807-8180268-33691476-5365
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