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A simple route to s...
A simple route to single-nucleotide polymorphisms in a nonmodel species : identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida)
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- Olsen, Morten Tange (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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Volny, Veronica Hirsh (författare)
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- Berube, Martine (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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Dietz, Rune (författare)
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Lydersen, Christian (författare)
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Kovacs, Kit M. (författare)
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Dodd, Richard S. (författare)
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- Palsbøll, Per (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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(creator_code:org_t)
- 2011-01-10
- 2011
- Engelska.
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Ingår i: Molecular Ecology Resources. - : Wiley. - 1755-098X .- 1755-0998. ; 11, s. 9-19
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Although single-nucleotide polymorphisms (SNPs) have become the marker of choice in the field of human genetics, these markers are only slowly emerging in ecological, evolutionary and conservation genetic analyses of nonmodel species. This is partly because of difficulties associated with the discovery and characterization of SNP markers. Herein, we adopted a simple straightforward approach to identifying SNPs, based on screening of a random genomic library. In total, we identified 768 SNPs in the ringed seal, Pusa hispida hispida, in samples from Greenland and Svalbard. Using three seal samples, SNPs were discovered at a rate of one SNP per 402 bp, whereas re-sequencing of 96 seals increased the density to one SNP per 29 bp. Although applicable to any species of interest, the approach is especially well suited for SNP discovery in nonmodel organisms and is easily implemented in any standard genetics laboratory, circumventing the need for prior genomic data and use of next-generation sequencing facilities.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
Nyckelord
- Arctic
- discovery
- genomics
- natural populations
- single-nucleotide polymorphism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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