Sökning: onr:"swepub:oai:DiVA.org:su-81820" >
A dynamic c termina...
A dynamic c terminal segment in the mycobacterium tuberculosis mn/fe r2lox protein can adopt a helical structure with possible functional consequences
-
- Andersson, Charlotta S., 1979- (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Berthold, Catrine L. (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Högbom, Martin (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
(creator_code:org_t)
- 2012-09-13
- 2012
- Engelska.
-
Ingår i: Chemistry and Biodiversity. - : Wiley. - 1612-1872 .- 1612-1880. ; 9:9, s. 1981-1988
- Relaterad länk:
-
https://su.diva-port... (primary) (Raw object)
-
visa fler...
-
http://su.diva-porta...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Mycobacterium tuberculosis R2-like ligand-binding oxidase (MtR2lox) belongs to a recently discovered group of proteins that are homologous to the ribonucleotide reductase R2 proteins. MtR2lox carries a heterodinuclear Mn/Fe cofactor and, unlike R2 proteins, a large ligand-binding cavity. A unique tyrosine-valine cross link is also found in the vicinity of the active site. To date, all known structures of R2 and R2lox proteins show a disordered C-terminal segment. Here, we present two new crystal forms of MtR2lox, revealing an ordered helical C-terminal. The ability of alternating between an ordered and disordered state agrees well with bioinformatic analysis of the protein sequence. Interestingly, ordering of the C-terminal helix shields a large positively charged patch on the protein surface, potentially used for interaction with other cellular components. We hypothesize that the dynamic C-terminal segment may be involved in control of protein function in vivo.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Nyckelord
- Protein folding
- Metalloproteins
- Structural dynamics
- Ribonucleotide reductases
- Oxidases
- X-Ray crystallography
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas