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Sökning: onr:"swepub:oai:DiVA.org:umu-139039" > B cells in tumor dr...

  • Zirakzadeh, A. Ali (författare)

B cells in tumor draining lymph nodes act asefficient antigen presenting cells in cancer patients

  • Artikel/kapitelEngelska2015

Förlag, utgivningsår, omfång ...

  • BioMed Central,2015
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:umu-139039
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-139039URI
  • https://doi.org/10.1186/2051-1426-3-S2-P65DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Introduction: Overall Survival of patients with muscle invasive urothelial bladder cancer MIBC remains around 50% (5 years), albeit some improvements by combining neoadjuvant chemotherapy with radical surgery. Our previous work has demonstrated that in vitro expansions of sentinel node-acquired autologous tumor specific CD4+ T cells are promising for adoptive immunotherapy [1]. In order for naive T helper cells to become activated, they need effective APCs, presenting tumor antigens. In another study, we observed that B cells in cancer patients were tumor antigen experienced and from their phenotypes we suggested a CD4+ T cell dependent anti-tumoral response [2]. In this study, we report a flow cytometric investigation of tumor draining lymph node (sentinel node) derived B cell activation by autologous tumor extract in patients with MIBC.Methods: Sentinel nodes (SNs) from 28 patients with MIBC were detected by a Geiger meter at cystectomy after peritumoral injection with radioactive isotope. Lymphocytes were isolated from freshly received SNs where they were stimulated with autologous tumor extract in a sterile environment. After cultivation for 7 days, the cells were analyzed by multi-color flow cytometry using FASCIA (Flow cytometric Assay of Specific Cell-mediated Immune response in Activated whole blood).Results: Patients displayed an increased B cell activation in SNs after stimulation with autologous tumor extract compared to when SN acquired lymphocytes were stimulated with autologous extract of macroscopically non-malignant bladder. CD4+ T cells from SNs were activated and formed blasts after co-culture with SN acquired B cells in the presence of tumor antigen. However, CD4+ T cells were not activated and did not blast when co-cultured with B cells incubated with HLA-DR-blocking antibodies. This indicates antigen presenting ability of SN acquired B cells.Conclusions: We demonstrate sentinel node acquired B lymphocytes can be activated in culture upon stimulation with autologous tumor extract but not with extract of non-malignant epithelium of the bladder, after 7 days. Lower number of sentinel node acquired CD4+ T cells cultured with HLA-DR blocked CD19+ cells in presence of tumor antigen, indicate functional antigen presenting ability of B cells in sentinel nodes. The role of B cells as APCs in human T cell anti-tumoral response should be further explored, as well as their usefulness in adoptive immunotherapy.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Krantz, David (författare)
  • Winerdal, Malin (författare)
  • Lundgren, Christian (författare)
  • Hartana, Ciputra (författare)
  • Ahlén Bergman, Emma (författare)
  • Hansson, Johan (författare)
  • Holmström, Benny (författare)
  • Sidiki, Alexander (författare)
  • Vasko, JanosUmeå universitet,Patologi(Swepub:umu)jasvao04 (författare)
  • Johansson, Markus (författare)
  • Marits, Per (författare)
  • Sherif, AmirUmeå universitet,Urologi och andrologi(Swepub:umu)amsh0008 (författare)
  • Winqvist, Ola (författare)
  • Umeå universitetPatologi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Journal for ImmunoTherapy of Cancer: BioMed Central3:Suppl 22051-1426

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