Sökning: onr:"swepub:oai:DiVA.org:umu-139039" >
B cells in tumor dr...
-
Zirakzadeh, A. Ali
(författare)
B cells in tumor draining lymph nodes act asefficient antigen presenting cells in cancer patients
- Artikel/kapitelEngelska2015
Förlag, utgivningsår, omfång ...
-
BioMed Central,2015
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:umu-139039
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-139039URI
-
https://doi.org/10.1186/2051-1426-3-S2-P65DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Introduction: Overall Survival of patients with muscle invasive urothelial bladder cancer MIBC remains around 50% (5 years), albeit some improvements by combining neoadjuvant chemotherapy with radical surgery. Our previous work has demonstrated that in vitro expansions of sentinel node-acquired autologous tumor specific CD4+ T cells are promising for adoptive immunotherapy [1]. In order for naive T helper cells to become activated, they need effective APCs, presenting tumor antigens. In another study, we observed that B cells in cancer patients were tumor antigen experienced and from their phenotypes we suggested a CD4+ T cell dependent anti-tumoral response [2]. In this study, we report a flow cytometric investigation of tumor draining lymph node (sentinel node) derived B cell activation by autologous tumor extract in patients with MIBC.Methods: Sentinel nodes (SNs) from 28 patients with MIBC were detected by a Geiger meter at cystectomy after peritumoral injection with radioactive isotope. Lymphocytes were isolated from freshly received SNs where they were stimulated with autologous tumor extract in a sterile environment. After cultivation for 7 days, the cells were analyzed by multi-color flow cytometry using FASCIA (Flow cytometric Assay of Specific Cell-mediated Immune response in Activated whole blood).Results: Patients displayed an increased B cell activation in SNs after stimulation with autologous tumor extract compared to when SN acquired lymphocytes were stimulated with autologous extract of macroscopically non-malignant bladder. CD4+ T cells from SNs were activated and formed blasts after co-culture with SN acquired B cells in the presence of tumor antigen. However, CD4+ T cells were not activated and did not blast when co-cultured with B cells incubated with HLA-DR-blocking antibodies. This indicates antigen presenting ability of SN acquired B cells.Conclusions: We demonstrate sentinel node acquired B lymphocytes can be activated in culture upon stimulation with autologous tumor extract but not with extract of non-malignant epithelium of the bladder, after 7 days. Lower number of sentinel node acquired CD4+ T cells cultured with HLA-DR blocked CD19+ cells in presence of tumor antigen, indicate functional antigen presenting ability of B cells in sentinel nodes. The role of B cells as APCs in human T cell anti-tumoral response should be further explored, as well as their usefulness in adoptive immunotherapy.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Krantz, David
(författare)
-
Winerdal, Malin
(författare)
-
Lundgren, Christian
(författare)
-
Hartana, Ciputra
(författare)
-
Ahlén Bergman, Emma
(författare)
-
Hansson, Johan
(författare)
-
Holmström, Benny
(författare)
-
Sidiki, Alexander
(författare)
-
Vasko, JanosUmeå universitet,Patologi(Swepub:umu)jasvao04
(författare)
-
Johansson, Markus
(författare)
-
Marits, Per
(författare)
-
Sherif, AmirUmeå universitet,Urologi och andrologi(Swepub:umu)amsh0008
(författare)
-
Winqvist, Ola
(författare)
-
Umeå universitetPatologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Journal for ImmunoTherapy of Cancer: BioMed Central3:Suppl 22051-1426
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Zirakzadeh, A. A ...
-
Krantz, David
-
Winerdal, Malin
-
Lundgren, Christ ...
-
Hartana, Ciputra
-
Ahlén Bergman, E ...
-
visa fler...
-
Hansson, Johan
-
Holmström, Benny
-
Sidiki, Alexande ...
-
Vasko, Janos
-
Johansson, Marku ...
-
Marits, Per
-
Sherif, Amir
-
Winqvist, Ola
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Urologi och njur ...
- Artiklar i publikationen
-
Journal for Immu ...
- Av lärosätet
-
Umeå universitet