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Urea dilution of se...
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
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- Olsson, Jan (författare)
- Umeå universitet,Virologi,Umeå Univ, Dept Clin Microbiol, Virol, Umeå, Sweden
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- Johansson, Jörgen (författare)
- Umeå universitet,Virologi,Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden; Umeå Univ Hosp, Lab Med, Clin Microbiol, Umeå, Sweden
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- Honkala, Emma (författare)
- Umeå universitet,Virologi,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden
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- Blomqvist, Bert (författare)
- Umeå universitet,Virologi,Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden; Umeå Univ Hosp, Lab Med, Clin Microbiol, Umeå, Sweden
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- Kok, Eloise (författare)
- Tampere Univ Hosp, Tampere, Finland
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- Weidung, Bodil (författare)
- Uppsala universitet,Geriatrik
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- Lövheim, Hugo, 1981- (författare)
- Umeå universitet,Geriatrik,Umeå Univ, Community Med & Rehabil, Geriatr Med, Umeå, Sweden
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- Elgh, Fredrik, 1957- (författare)
- Umeå universitet,Virologi,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden
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(creator_code:org_t)
- 2019-02-14
- 2019
- Engelska.
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Ingår i: BMC Infectious Diseases. - : BioMed Central. - 1471-2334. ; 19
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://bmcinfectdis...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900-0.979) and 0.989 (95% CI 0.969-0.996) in the biobank and clinical samples, respectively. The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- Herpes simplex
- IgG
- Avidity
- ELISA
- Primary infection
- Reactivated infection
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Olsson, Jan
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Johansson, Jörge ...
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Honkala, Emma
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Blomqvist, Bert
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Kok, Eloise
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Weidung, Bodil
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Lövheim, Hugo, 1 ...
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Elgh, Fredrik, 1 ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
- Artiklar i publikationen
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BMC Infectious D ...
- Av lärosätet
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Umeå universitet
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Uppsala universitet