Simultaneous genotyping of all hemagglutinin and neuraminidase subtypes of avian influenza viruses by use of padlock probes

A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.

Conze, TimUppsala universitet,Molekylära verktyg(Swepub:uu)ticon367 (författare)
Zohari, Siamak (författare)
LeBlanc, Neil (författare)
Nilsson, MatsUppsala universitet,Molekylära verktyg(Swepub:uu)matsnils (författare)
Landegren, UlfUppsala universitet,Molekylära verktyg(Swepub:uu)ulfland (författare)
Banér, Johan (författare)
Belák, Sándor (författare)
Uppsala universitetMolekylära verktyg (creator_code:org_t)

Av författaren/redakt...: Gyarmati, Péter; Conze, Tim; Zohari, Siamak; LeBlanc, Neil; Nilsson, Mats; Landegren, Ulf; visa fler...; Banér, Johan; Belák, Sándor; visa färre...

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