Sökning: onr:"swepub:oai:DiVA.org:uu-169911" >
Sensitive detection...
Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry
-
Dahlén, P (författare)
-
Syvänen, Ann-Christine (författare)
-
Hurskainen, P (författare)
-
visa fler...
-
Kwiatkowski, M (författare)
-
Sund, C (författare)
-
Ylikoski, J (författare)
-
Söderlund, H (författare)
-
Lövgren, T (författare)
-
visa färre...
- Elsevier BV, 1987
- 1987
- Engelska.
-
Ingår i: Molecular and Cellular Probes. - : Elsevier BV. - 0890-8508 .- 1096-1194. ; 1:2, s. 159-168
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Europium has been used as a non-radioactive marker in immunoassays as this metal can be detected with high sensitivity by time-resolved fluorometry. In this work streptavidin labeled with europium was used to detect biotinylated probes in a sandwich nucleic-acid hybridization assay with microtitration strips as the solid phase. pBR 322 plasmids were detected with a sensitivity of 4 × 105 molecules. As the sample is added in solution in sandwich hybridization, fast and simple sample pre-treatment can be used without encountering background problems. The method was applied to test bacterial samples of uropathogenic Escherichia coli strains for the presence of the β-lactamase gene.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas