Deciphering complex protein interaction kinetics using Interaction Map

Altschuh, DanièleBiotechnologie et signalisation cellulaire, Université de Strasbourg, France (författare)

Cellular receptor systems are expected to present complex ligand interaction patterns that cannot beevaluated assuming a simple one ligand:one receptor interaction model. We have previously evaluatedheterogeneous interactions using an alternative method to regression analysis, called Interaction Map(IM). IM decomposes a time-resolved binding curve into its separate components. By replacing the reductionistic,scalar kinetic association rate constant ka and dissociation rate constant kd with a two-dimensionaldistribution of ka and kd, it is possible to display heterogeneous data as a map where each peakcorresponds to one of the components that contribute to the cumulative binding curve. Here we challengethe Interaction Map approach by artificially generating heterogeneous data from two known interactions,on either LigandTracer or Surface Plasmon Resonance devices. We prove the ability of IM toaccurately decompose these man-made heterogeneous binding curves composed of two different interactions.We conclude that the Interaction Map approach is well suited for the analysis of complex bindingdata and forecast that it has a potential to resolve previously uninterpretable data, in particular thosegenerated in cell-based assays.

Björkelund, HannaUppsala universitet,Enheten för biomedicinsk strålningsvetenskap(Swepub:uu)hanbj985 (författare)
Strandgård, JohnRidgeview Instruments AB, Uppsala, Sweden(Swepub:uu)johst912 (författare)
Chouliera, LaurenceBiotechnologie et signalisation cellulaire, Université de Strasbourg, France (författare)
Malmqvist, MagnusUppsala universitet,Enheten för biomedicinsk strålningsvetenskap(Swepub:uu)magma412 (författare)
Andersson, KarlUppsala universitet,Enheten för biomedicinsk strålningsvetenskap(Swepub:uu)kaand227 (författare)
Biotechnologie et signalisation cellulaire, Université de Strasbourg, FranceEnheten för biomedicinsk strålningsvetenskap (creator_code:org_t)

Ingår i:Biochemical and Biophysical Research Communications - BBRC: Elsevier BV428:1, s. 74-790006-291X1090-2104

Av författaren/redakt...: Altschuh, Danièl ...; Björkelund, Hann ...; Strandgård, John; Chouliera, Laure ...; Malmqvist, Magnu ...; Andersson, Karl

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