Sökning: onr:"swepub:oai:DiVA.org:uu-239670" >
Altered microRNA pr...
Altered microRNA profiles in bronchoalveolar lavage fluid exosomes in asthmatic patients.
-
- Levänen, Bettina (författare)
- Karolinska Institutet
-
Bhakta, Nirav R (författare)
-
Torregrosa Paredes, Patricia (författare)
-
visa fler...
-
Barbeau, Rebecca (författare)
-
Hiltbrunner, Stefanie (författare)
-
Pollack, Joshua L (författare)
-
- Sköld, C Magnus (författare)
- Karolinska Institutet
-
- Svartengren, Magnus (författare)
- Karolinska Institutet,Uppsala universitet,Arbets- och miljömedicin,Eva Vingård
-
- Grunewald, Johan (författare)
- Karolinska Institutet
-
- Gabrielsson, Susanne (författare)
- Karolinska Institutet
-
Eklund, Anders (författare)
-
- Larsson, Britt-Marie (författare)
- Karolinska Institutet
-
Woodruff, Prescott G (författare)
-
Erle, David J (författare)
-
- Wheelock, Åsa M (författare)
- Karolinska Institutet
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2013
- 2013
- Engelska.
-
Ingår i: Journal of Allergy and Clinical Immunology. - : Elsevier BV. - 0091-6749 .- 1097-6825. ; 131:3, s. 894-903
- Relaterad länk:
-
https://europepmc.or...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- BACKGROUND: Asthma is characterized by increased airway narrowing in response to nonspecific stimuli. The disorder is influenced by both environmental and genetic factors. Exosomes are nanosized vesicles of endosomal origin released from inflammatory and epithelial cells that have been implicated in asthma. In this study we characterized the microRNA (miRNA) content of exosomes in healthy control subjects and patients with mild intermittent asthma both at unprovoked baseline and in response to environmental challenge.OBJECTIVE: To investigate alterations in bronchoalveolar lavage fluid (BALF) exosomal miRNA profiles due to asthma, and following subway air exposure.METHODS: Exosomes were isolated from BALF from healthy control subjects (n = 10) and patients with mild intermittent asthma (n = 10) after subway and control exposures. Exosomal RNA was analyzed by using microarrays containing probes for 894 human miRNAs, and selected findings were validated with quantitative RT-PCR. Results were analyzed by using multivariate modeling.RESULTS: The presence of miRNAs was confirmed in exosomes from BALF of both asthmatic patients and healthy control subjects. Significant differences in BALF exosomal miRNA was detected for 24 miRNAs with a subset of 16 miRNAs, including members of the let-7 and miRNA-200 families, providing robust classification of patients with mild nonsymptomatic asthma from healthy subjects with 72% cross-validated predictive power (Q(2) = 0.72). In contrast, subway exposure did not cause any significant alterations in miRNA profiles.CONCLUSION: These studies demonstrate substantial differences in exosomal miRNA profiles between healthy subjects and patients with unprovoked, mild, stable asthma. These changes might be important in the inflammatory response leading to bronchial hyperresponsiveness and asthma.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Arbetsmedicin och miljömedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Occupational Health and Environmental Health (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Levänen, Bettina
-
Bhakta, Nirav R
-
Torregrosa Pared ...
-
Barbeau, Rebecca
-
Hiltbrunner, Ste ...
-
Pollack, Joshua ...
-
visa fler...
-
Sköld, C Magnus
-
Svartengren, Mag ...
-
Grunewald, Johan
-
Gabrielsson, Sus ...
-
Eklund, Anders
-
Larsson, Britt-M ...
-
Woodruff, Presco ...
-
Erle, David J
-
Wheelock, Åsa M
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Hälsovetenskap
-
och Arbetsmedicin oc ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Lungmedicin och ...
- Artiklar i publikationen
-
Journal of Aller ...
- Av lärosätet
-
Uppsala universitet
-
Karolinska Institutet