Sökning: onr:"swepub:oai:DiVA.org:uu-245040" > Imaging single cell...
Fältnamn | Indikatorer | Metadata |
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000 | 04694naa a2200757 4500 | |
001 | oai:DiVA.org:uu-245040 | |
003 | SwePub | |
008 | 150224s2015 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2450402 URI |
024 | 7 | a https://doi.org/10.1038/ncomms67042 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a van der Schot, Gijsu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)gijva150 |
245 | 1 0 | a Imaging single cells in a beam of live cyanobacteria with an X-ray laser |
264 | c 2015-02-11 | |
264 | 1 | b Springer Science and Business Media LLC,c 2015 |
338 | a print2 rdacarrier | |
520 | a There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential. | |
650 | 7 | a NATURVETENSKAPx Biologix Strukturbiologi0 (SwePub)106012 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Structural Biology0 (SwePub)106012 hsv//eng |
700 | 1 | a Svenda, Martinu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)msv31659 |
700 | 1 | a Maia, Filipe R. N. C.u Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)fimai103 |
700 | 1 | a Hantke, Maxu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)maxha207 |
700 | 1 | a DePonte, Daniel P.4 aut |
700 | 1 | a Seibert, M. Marvinu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)masei255 |
700 | 1 | a Aquila, Andrew4 aut |
700 | 1 | a Schulz, Joachim4 aut |
700 | 1 | a Kirian, Richard4 aut |
700 | 1 | a Liang, Mengning4 aut |
700 | 1 | a Stellato, Francesco4 aut |
700 | 1 | a Iwan, Biancau Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)biaiw419 |
700 | 1 | a Andreasson, Jakobu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)jakan998 |
700 | 1 | a Timneanu, Nicusoru Uppsala universitet,Molekylär biofysik,Molekyl- och kondenserade materiens fysik4 aut0 (Swepub:uu)nti19847 |
700 | 1 | a Westphal, Danielu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)danwe345 |
700 | 1 | a Almeida, F. Nunesu Uppsala universitet,Molekylär biofysik4 aut |
700 | 1 | a Odic, Duskou Uppsala universitet,Molekylär biofysik4 aut |
700 | 1 | a Hasse, Dirku Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)dirha931 |
700 | 1 | a Carlsson, Gunilla H.u Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)gca29842 |
700 | 1 | a Larsson, Daniel S. D.u Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)dalar193 |
700 | 1 | a Barty, Anton4 aut |
700 | 1 | a Martin, Andrew V.4 aut |
700 | 1 | a Schorb, Sebastian4 aut |
700 | 1 | a Bostedt, Christoph4 aut |
700 | 1 | a Bozek, John D.4 aut |
700 | 1 | a Rolles, Daniel4 aut |
700 | 1 | a Rudenko, Artem4 aut |
700 | 1 | a Epp, Sascha4 aut |
700 | 1 | a Foucar, Lutz4 aut |
700 | 1 | a Rudek, Benedikt4 aut |
700 | 1 | a Hartmann, Robert4 aut |
700 | 1 | a Kimmel, Nils4 aut |
700 | 1 | a Holl, Peter4 aut |
700 | 1 | a Englert, Lars4 aut |
700 | 1 | a Duane Loh, Ne-Te4 aut |
700 | 1 | a Chapman, Henry N.4 aut |
700 | 1 | a Andersson, Ingeru Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)inand172 |
700 | 1 | a Hajdu, Janosu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)janohajd |
700 | 1 | a Ekeberg, Tomasu Uppsala universitet,Molekylär biofysik4 aut0 (Swepub:uu)tomek611 |
710 | 2 | a Uppsala universitetb Molekylär biofysik4 org |
773 | 0 | t Nature Communicationsd : Springer Science and Business Media LLCg 6q 6x 2041-1723 |
856 | 4 | u https://www.nature.com/articles/ncomms6704.pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-245040 |
856 | 4 8 | u https://doi.org/10.1038/ncomms6704 |
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