Sökning: onr:"swepub:oai:DiVA.org:uu-264600" >
Role of the AMP kin...
Role of the AMP kinase in cytokine-induced human EndoC-beta H1 cell death
-
- Fred, Rikard G. (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab
-
- Kappe, Camilla (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk cellbiologi
-
- Ameur, Adam (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
-
visa fler...
-
- Cen, Jing (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk cellbiologi
-
- Bergsten, Peter (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab
-
- Ravassard, Phillippe (författare)
- Univ Paris 06, Univ Paris 04, Biotechnol & Biotherapy Lab,Inst Cerveau & Moelle, Inserm,U1127,CNRS UMR 7225,UMR S 1127,ICM,CHU Pit, Paris, France.
-
- Scharfmann, Raphael (författare)
- Univ Paris 05, Sorbonne Paris Cite, Fac Med, INSERM,U1016,Inst Cochin, F-75014 Paris, France.
-
- Welsh, Nils (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk cellbiologi
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- Engelska.
-
Ingår i: Molecular and Cellular Endocrinology. - : Elsevier BV. - 0303-7207 .- 1872-8057. ; 414:C, s. 53-63
- Relaterad länk:
-
https://uu.diva-port... (primary) (Raw object)
-
visa fler...
-
http://uu.diva-porta...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The aim of the present investigation was to delineate cytokine-induced signaling and death using the EndoC-beta H1 cells as a model for primary human beta-cells. The cytokines IL-1 beta and IFN-gamma induced a rapid and transient activation of NF-kappa B, STAT-1, ERK, JNK and eIF-2 alpha signaling. The EndoC-beta H1 cells died rapidly when exposed to IL-1 beta + IFN-gamma, and this occurred also in the presence of the actinomycin D. Inhibition of NF-kappa B and STAT-1 did not protect against cell death, nor did the cytokines activate iNOS expression. Instead, cytokines promoted a rapid decrease in EndoC-beta H1 cell respiration and ATP levels, and we observed protection by the AMPK activator AICAR against cytokine-induced cell death. It is concluded that EndoC-beta H1 cell death can be prevented by AMPK activation, which suggests a role for ATP depletion in cytokine-induced human beta-cell death.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- AMPK
- ATP
- EndoC-beta H1 cells
- Cytokines
- Apoptosis
- NF-kappaB
- STAT-1
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas