onr:"swepub:oai:DiVA.org:uu-277613"
Sökning: onr:"swepub:oai:DiVA.org:uu-277613" > Mass Spectrometry-b...
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
- Musunuri, SravaniUppsala universitet,Analytisk kemi,Uppsala University (författare)
Mass Spectrometry-based Neuroproteomics : Deciphering the Human Brain Proteome
- BokEngelska2016
Förlag, utgivningsår, omfång ...
- Uppsala :Acta Universitatis Upsaliensis,2016
- 70 s.
- electronicrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:uu-277613
- ISBN:9789155494858
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-277613URI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Serie
- Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,1651-6214 ;1347
Anmärkningar
- Mammalian brain is challenging to study due to its heterogeneity and complexity. However, recent advances in molecular imaging, genomics and proteomics have contributed significantly to achieve insights into molecular basis of brain function and pathogenesis of neurological disorders. Efficient sample preparation is an integral part of a successful mass spectrometry (MS)-based proteomics. Apart from the identification, quantification of proteins is needed to investigate the alterations between proteome profiles from different sample sets. Therefore, this thesis investigates optimizing and application of the MS compatible sample preparation techniques for the identification and quantification of proteins from brain tissue.The central objective of this thesis was (i) to improve the extraction of proteins as well as membrane proteins (MPs) from the brain tissue and (ii) to apply the optimized method along with the stable isotope dimethyl labeling (DML) and label-free (LF) MS approaches for the relative quantification of the brain proteome profiles during neurological conditions such as Alzheimer’s disease (AD) and traumatic brain injury (TBI). First study described in this thesis is focused on the qualitative aspects for the brain tissue sample preparation. The optimized extraction buffers from first study containing n-octyl-β-glucopyranside or triton X-114 were used in the further quantitative studies to extract the proteins from patient (AD or TBI) and control human brain samples. Triton X-114 has additional advantage of separating MPs into a micellar phase. Therefore we also investigated the possibility to apply this in combination with DML quantitation approach for enrichment of low abundant MPs from AD brains.AD and TBI causes severe socio-economic burden on the society and therefore there is a need to develop diagnostic markers to detect the early changes in the pathology of the disease. Analytical tools and techniques applied and discussed in this thesis for neuroproteomics applications proved to be powerful and reliable for analyzing complex biological samples to generate high-throughput screening and unbiased identification and quantitation of disease-specific proteins that are of great importance in understanding the disease pathology.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Kemi Analytisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
- Brain
- Proteomics
- Mass spectrometry
- Alzheimer's disease
- Traumatic brain injury
- Membrane proteins
- Sample preparation
- Kemi med inriktning mot analytisk kemi
- Chemistry with specialization in Analytical Chemistry
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Bergquist, Jonas,ProfessorUppsala universitet,Analytisk kemi (preses)
- Shevchenko, Ganna,DrUppsala universitet,Analytisk kemi (preses)
- Sanchez, Jean-Charles,ProfessorUniversity of Geneva (opponent)
- Uppsala universitetAnalytisk kemi (creator_code:org_t)
Internetlänk
Hitta via bibliotek
- Mass Spectrometry-based Neuroproteomics Deciphering the Human Brain Proteome (Sök publikationen i LIBRIS)
Till lärosätets databas
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Musunuri, Sravan ...
- Bergquist, Jonas ...
- Shevchenko, Gann ...
- Sanchez, Jean-Ch ...
- Om ämnet
-
- NATURVETENSKAP
- NATURVETENSKAP
- och Kemi
- och Analytisk kemi
- Delar i serien
- Digital Comprehe ...
- Av lärosätet
- Uppsala universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
