SwePub
Sök i LIBRIS databas

  Utökad sökning

onr:"swepub:oai:DiVA.org:uu-297800"
 

Sökning: onr:"swepub:oai:DiVA.org:uu-297800" > A Novel HLA-DRB1*10...

  • Chemin, KarineKarolinska Institutet (författare)

A Novel HLA-DRB1*10:01-Restricted T Cell Epitope From Citrullinated Type II Collagen Relevant to Rheumatoid Arthritis

  • Artikel/kapitelEngelska2016

Förlag, utgivningsår, omfång ...

  • Wiley,2016
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-297800
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297800URI
  • https://doi.org/10.1002/art.39553DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:133495847URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Objective. Antibodies against citrullinated type II collagen (Cit-CII) are common in the sera and synovial fluid of patients with rheumatoid arthritis (RA); however, the known T cell epitope of CII is not dependent on citrullination. The aim of this study was to identify and functionally characterize the Cit-CII-restricted T cell epitopes that are relevant to RA. Methods. Peripheral blood mononuclear cells (PBMCs) from HLA-DRB1*10:01-positive patients with RA and healthy donors were stimulated in vitro with candidate CII peptides. CD154 up-regulation was measured as a marker of antigen-specific activation, and anti-HLA-DR-blocking experiments confirmed HLA restriction. Cytokine production was measured using a Luminex technique. Direct peptide-binding assays using HLA-DRB1*10:01 and HLA-DRB1*04:01 monomeric proteins were performed. The T cell receptor (TCR) beta-chain of CD154-enriched antigen-specific T cells was analyzed using high-throughput sequencing. Results. A novel Cit-CII peptide was identified based on its ability to activate CD4+ T cells from HLA-DRB1*10:01-positive individuals. When stimulated in vitro, Cit-CII autoreactive T cells produced proinflammatory cytokines. Cit-CII311-325 bound (with low affinity) to HLA-DRB1*10:01 but not to HLA-DRB1*04:01, while the native form was unable to bind either protein. In addition, highly expanded clones were identified in the TCR beta repertoire of Cit-CII311-325-stimulated PBMCs. Conclusion. These results illustrate the ability of the citrullination process to create T cell epitopes from CII, a cartilage-restricted protein that is relevant to RA pathogenesis. The exclusive binding of Cit-CII311-325 to HLA-DRB1*10:01 suggests that recognition of citrullinated epitopes might vary between individuals carrying different RA-associated HLA-DR molecules.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Pollastro, SabrinaAmsterdam Rheumatol & Immunol Ctr, Amsterdam, Netherlands.;Univ Amsterdam, Acad Med Ctr, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands. (författare)
  • James, EddieVirginia Mason, Benaroya Res Inst, Seattle, WA USA. (författare)
  • Ge, ChangrongKarolinska Institutet (författare)
  • Albrecht, InkaKarolinska Univ Hosp, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden. (författare)
  • Herrath, JessicaKarolinska Univ Hosp, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden. (författare)
  • Gerstner, ChristinaKarolinska Institutet (författare)
  • Tandre, Karolina (författare)
  • Rizzi, Thais SampaioAmsterdam Rheumatol & Immunol Ctr, Amsterdam, Netherlands.;Univ Amsterdam, Acad Med Ctr, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands. (författare)
  • Rönnblom, LarsUppsala universitet,Reumatologi(Swepub:uu)larsronn (författare)
  • Catrina, AncaKarolinska Institutet (författare)
  • Holmdahl, RikardKarolinska Institutet (författare)
  • Klareskog, LarsKarolinska Institutet (författare)
  • de Vries, NiekAmsterdam Rheumatol & Immunol Ctr, Amsterdam, Netherlands.;Univ Amsterdam, Acad Med Ctr, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands. (författare)
  • Malmstrom, VivianneKarolinska Institutet (författare)
  • Karolinska InstitutetAmsterdam Rheumatol & Immunol Ctr, Amsterdam, Netherlands.;Univ Amsterdam, Acad Med Ctr, Meibergdreef 9, NL-1105 AZ Amsterdam, Netherlands. (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Arthritis & Rheumatology: Wiley68:5, s. 1124-11352326-51912326-5205

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy