Sökning: onr:"swepub:oai:DiVA.org:uu-390531" >
The NeuroD6 Subtype...
The NeuroD6 Subtype of VTA Neurons Contributes to Psychostimulant Sensitization and Behavioral Reinforcement
-
- Bimpisidis, Zisis (författare)
- Uppsala universitet,Jämförande fysiologi
-
- König, Niclas, 1986- (författare)
- Uppsala universitet,Jämförande fysiologi
-
- Stagkourakis, Stefanos (författare)
- Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden
-
visa fler...
-
- Zell, Vivien (författare)
- Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA
-
- Vlcek, Bianca (författare)
- Uppsala universitet,Jämförande fysiologi
-
- Dumas, Sylvie (författare)
- Oramacell, 8 Rue Gregoire Tours, F-75006 Paris, France
-
- Giros, Bruno (författare)
- INSERM, UMRS 1130, F-75005 Paris, France;CNRS, Unite Mixte Rech 8246, F-75005 Paris, France;Univ Paris 06, Sorbonne Univ, Neurosci Paris Seine, F-75005 Paris, France;Douglas Mental Hlth Univ Inst, 6875 LaSalle Blvd, Verdun, PQ H4H 1R3, Canada;McGill Univ, Dept Psychiat, Montreal, PQ, Canada
-
- Broberger, Christian (författare)
- Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden
-
- Hnasko, Thomas S. (författare)
- Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA;Res Serv VA San Diego Healthcare Syst, La Jolla, CA 92161 USA
-
- Wallén-Mackenzie, Åsa (författare)
- Uppsala universitet,Jämförande fysiologi
-
visa färre...
-
(creator_code:org_t)
- SOC NEUROSCIENCE, 2019
- 2019
- Engelska.
-
Ingår i: eNeuro. - : SOC NEUROSCIENCE. - 2373-2822. ; 6:3
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Reward-related behavior is complex and its dysfunction correlated with neuropsychiatric illness. Dopamine (DA) neurons of the ventral tegmental area (VTA) have long been associated with different aspects of reward function, but it remains to be disentangled how distinct VTA DA neurons contribute to the full range of behaviors ascribed to the VTA. Here, a recently identified subtype of VTA neurons molecularly defined by NeuroD6 (NEX1M) was addressed. Among all VTA DA neurons, less than 15% were identified as positive for NeuroD6. In addition to dopaminergic markers, sparse NeuroD6 neurons expressed the vesicular glutamate transporter 2 (Vglut2) gene. To achieve manipulation of NeuroD6 VTA neurons, NeuroD6(NEX)-Cre-driven mouse genetics and optogenetics were implemented. First, expression of vesicular monoamine transporter 2 (VMAT2) was ablated to disrupt dopaminergic function in NeuroD6 VTA neurons. Comparing Vmat2(Cre)(lox/lox;NEX-) conditional knock-out (cKO) mice with littermate controls, it was evident that baseline locomotion, preference for sugar and ethanol, and place preference upon amphetamine-induced and cocaine-induced conditioning were similar between genotypes. However, locomotion upon repeated psychostimulant administration was significantly elevated above control levels in cKO mice. Second, optogenetic activation of NEX-Cre VTA neurons was shown to induce DA release and glutamatergic postsynaptic currents within the nucleus accumbens. Third, optogenetic stimulation of NEX-Cre VTA neurons in vivo induced significant place preference behavior, while stimulation of VTA neurons defined by Calretinin failed to cause a similar response. The results show that NeuroD6 VTA neurons exert distinct regulation over specific aspects of reward-related behavior, findings that contribute to the current understanding of VTA neurocircuitry.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Nyckelord
- accumbens
- dopamine
- mouse genetics
- optogenetics
- reward
- ventral tegmental area
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
eNeuro
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- Av författaren/redakt...
-
Bimpisidis, Zisi ...
-
König, Niclas, 1 ...
-
Stagkourakis, St ...
-
Zell, Vivien
-
Vlcek, Bianca
-
Dumas, Sylvie
-
visa fler...
-
Giros, Bruno
-
Broberger, Chris ...
-
Hnasko, Thomas S ...
-
Wallén-Mackenzie ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Neurovetenskaper
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Neurologi
- Artiklar i publikationen
-
eNeuro
- Av lärosätet
-
Uppsala universitet
-
Karolinska Institutet