Sökning: onr:"swepub:oai:DiVA.org:uu-431535" > Evaluation of NTRK ...
Fältnamn | Indikatorer | Metadata |
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000 | 06885naa a2200709 4500 | |
001 | oai:DiVA.org:uu-431535 | |
003 | SwePub | |
008 | 210114s2021 | |||||||||||000 ||eng| | |
009 | oai:lup.lub.lu.se:f1878477-5df8-4318-9ea2-9691604e6239 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4315352 URI |
024 | 7 | a https://doi.org/10.1016/j.lungcan.2020.11.0232 DOI |
024 | 7 | a https://lup.lub.lu.se/record/f1878477-5df8-4318-9ea2-9691604e62392 URI |
040 | a (SwePub)uud (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Elfving, Hedvigu Uppsala University,Uppsala universitet,Institutionen för immunologi, genetik och patologi,Patrick Micke4 aut0 (Swepub:uu)hedel331 |
245 | 1 0 | a Evaluation of NTRK immunohistochemistry as a screening method for NTRK gene fusion detection in non-small cell lung cancer |
264 | 1 | b Elsevier,c 2021 |
338 | a print2 rdacarrier | |
500 | a De två första författarna delar förstaförfattarskapet | |
520 | a Purpose: The small molecule inhibitors larotrectinib and entrectinib have recently been approved as cancer agnostic drugs in patients with tumours harbouring a rearrangement of the neurotrophic tropomyosin receptor kinase (NTRK). These oncogenic fusions are estimated to occur in 0.1-3 % of non-small cell lung cancers (NSCLC). Although molecular techniques are most reliable for fusion detection, immunohistochemical analysis is considered valuable for screening. Therefore, we evaluated the newly introduced diagnostic immunohistochemical assay (clone EPR17341) on a representative NSCLC cohort.Methods: Cancer tissue from 688 clinically and molecularly extensively annotated NSCLC patients were comprised on tissue microarrays and stained with the pan-TRK antibody clone EPR17341. Positive cases were further analysed with the TruSight Tumor 170 RNA assay (Illumina). Selected cases were also tested with a NanoString NTRK fusion assay. For 199 cases, NTRK RNA expression data were available from previous RNA sequencing analysis.Results: Altogether, staining patterns for 617 NSCLC cases were evaluable. Of these, four cases (0.6 %) demonstrated a strong diffuse cytoplasmic and membranous staining, and seven cases a moderate staining (1.1 %). NanoString or TST170-analysis could not confirm an NTRK fusion in any of the IHC positive cases, or any of the cases with high mRNA levels. In the four cases with strong staining intensity in the tissue microarray, whole section staining revealed marked heterogeneity of NTRK protein expression.Conclusion: The presence of NTRK fusion genes in non-small cell lung cancer is exceedingly rare. The use of the immunohistochemical NTRK assay will result in a small number of false positive cases. This should be considered when the assay is applied as a screening tool in clinical diagnostics. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Klinisk laboratoriemedicin0 (SwePub)302232 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Clinical Laboratory Medicine0 (SwePub)302232 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
653 | a Diagnostics | |
653 | a Entrectinib | |
653 | a Gene fusion | |
653 | a Larotrectinib | |
653 | a Lung adenocarcinoma | |
653 | a NTRK | |
653 | a Pathology | |
653 | a Patologi | |
653 | a Diagnostics | |
653 | a Entrectinib | |
653 | a Gene fusion | |
653 | a Larotrectinib | |
653 | a Lung adenocarcinoma | |
653 | a NTRK | |
700 | 1 | a Broström, Erikau Uppsala University,Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)eribr175 |
700 | 1 | a Moens, Lotte N.J.u Uppsala University,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Johan Botling4 aut0 (Swepub:uu)lotmo617 |
700 | 1 | a Almlöf, Jonasu Uppsala University,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)jonca820 |
700 | 1 | a Cerjan, Dijanau Uppsala University,Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)dijce981 |
700 | 1 | a Lauter, Gilbertu Uppsala University,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Johan Botling4 aut0 (Swepub:uu)gilla825 |
700 | 1 | a Nord, Helena,d 1980-u Uppsala University,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Johan Botling4 aut0 (Swepub:uu)helno987 |
700 | 1 | a Mattsson, Johanna S. M.,d 1985-u Uppsala University,Uppsala universitet,Institutionen för immunologi, genetik och patologi,Patrick Micke4 aut0 (Swepub:uu)johma961 |
700 | 1 | a Ullenhag, Gustav J.u Uppsala University,Uppsala universitet,Institutionen för immunologi, genetik och patologi,Uppsala University Hospital4 aut0 (Swepub:uu)gustulle |
700 | 1 | a Strell, Carinau Uppsala University4 aut0 (Swepub:uu)carst810 |
700 | 1 | a Backman, Maxu Uppsala University,Patrick Micke4 aut0 (Swepub:uu)maxba655 |
700 | 1 | a La Fleur, Linnéau Uppsala University,Uppsala universitet,Institutionen för immunologi, genetik och patologi,Johan Botling4 aut0 (Swepub:uu)linla513 |
700 | 1 | a Brunnström, Hansu Lund University,Lunds universitet,Förbättrad diagnostik och prognostik vid lungcancer och metastaser till lunga,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Improved diagnostics and prognostics of lung cancer and metastases to the lungs,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments,Skåne University Hospital4 aut0 (Swepub:lu)med-hsb |
700 | 1 | a Botling, Johanu Uppsala University,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Johan Botling4 aut0 (Swepub:uu)johanbot |
700 | 1 | a Micke, Patricku Uppsala University,Patrick Micke4 aut0 (Swepub:uu)patmi676 |
710 | 2 | a Uppsala universitetb Institutionen för immunologi, genetik och patologi4 org |
773 | 0 | t Lung Cancerd : Elsevierg 151, s. 53-59q 151<53-59x 0169-5002x 1872-8332 |
856 | 4 | u http://www.lungcancerjournal.info/article/S0169500220306978/pdf |
856 | 4 | u http://dx.doi.org/10.1016/j.lungcan.2020.11.023y FULLTEXT |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-431535 |
856 | 4 8 | u https://doi.org/10.1016/j.lungcan.2020.11.023 |
856 | 4 8 | u https://lup.lub.lu.se/record/f1878477-5df8-4318-9ea2-9691604e6239 |
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