Sökning: onr:"swepub:oai:DiVA.org:uu-72965" >
Proteomics-based id...
-
Grimsby, SusanneUppsala universitet,Ludwiginstitutet för cancerforskning
(författare)
Proteomics-based identification of proteins interacting with Smad3 : SREBP-2 forms a complex with Smad3 and inhibits its transcriptional activity
- Artikel/kapitelEngelska2004
Förlag, utgivningsår, omfång ...
-
2004-10-07
-
Wiley,2004
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:uu-72965
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-72965URI
-
https://doi.org/10.1016/j.febslet.2004.09.069DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:110654603URI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska &language:-1_t
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Smad3 is an important component of transforming growth factor-beta (TGFbeta) intracellular signalling. To identify novel interacting proteins of Smad3, we performed pull-down assays with Smad3 constructs fused to glutathione-S-transferase. Proteins which formed complexes with these constructs were analyzed by two-dimensional gel electrophoresis, and were identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. We identified 14 proteins interacting with the Smad3 construct lacking the N-terminal Mad homology domain 1 (MH1), and 12 proteins interacting with the construct lacking the C-terminal MH2 domain. Proteins involved in signalling processes, in metabolism regulation, novel proteins, and components of cytoskeleton form four groups of interacting proteins. Interactions of AGP7, sex-determining region Y protein, actin beta and sterol regulatory element binding protein-2 (SREBP-2) proteins with Smad3 constructs were confirmed by immunoblotting with specific antibodies. Interaction of Smad3 with SREBP-2 was also confirmed by co-immunoprecipitation of myc-Smad3 and Flag-SREBP-2 upon expression in mammalian cells. We found that SREBP-2 inhibited the transcriptional activity of Smad3 in luciferase reporter assays.
Ämnesord och genrebeteckningar
-
Animals
-
Cell Line
-
DNA-Binding Proteins/*metabolism
-
Electrophoresis; Gel; Two-Dimensional
-
Humans
-
Protein Binding
-
Proteome
-
Research Support; Non-U.S. Gov't
-
Spectrometry; Mass; Matrix-Assisted Laser Desorption-Ionization
-
Trans-Activators/*metabolism
-
Transcription Factors/*metabolism
-
Transcription; Genetic
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Jaensson, HannaUppsala universitet,Ludwiginstitutet för cancerforskning
(författare)
-
Dubrovska, AnnaUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)andub498
(författare)
-
Lomnytska, MartaUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)marlo829
(författare)
-
Hellman, UlfUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)ulfhm
(författare)
-
Souchelnytskyi, SerhiyKarolinska Institutet,Uppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)sergsouc
(författare)
-
Uppsala universitetLudwiginstitutet för cancerforskning
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:FEBS Letters: Wiley577:1-2, s. 93-1000014-57931873-3468
Internetlänk
Hitta via bibliotek
Till lärosätets databas