Sökning: onr:"swepub:oai:DiVA.org:uu-98774" >
The flavouring phyt...
The flavouring phytochemical 2-pentanone reduces prostaglandin production and COX-2 expression in colon cancer cells
-
- Pettersson, Jenny (författare)
- Uppsala universitet,Avdelningen för farmakognosi
-
Karlsson, Pernilla Christina (författare)
-
- Göransson, Ulf (författare)
- Uppsala universitet,Avdelningen för farmakognosi
-
visa fler...
-
- Rafter, Joseph James (författare)
- Karolinska Institutet
-
- Bohlin, Lars (författare)
- Uppsala universitet,Avdelningen för farmakognosi
-
visa färre...
-
(creator_code:org_t)
- Pharmaceutical Society of Japan, 2008
- 2008
- Engelska.
-
Ingår i: Biological and Pharmaceutical Bulletin. - : Pharmaceutical Society of Japan. - 0918-6158 .- 1347-5215. ; 31:3, s. 534-537
- Relaterad länk:
-
https://www.jstage.j...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Many phytochemicals found in the diet may prevent colon carcinogenesis by affecting biochemical processes in the colonic mucosa. Inflammation and subsequent elevation of the enzyme cyclooxygenase-2 (COX-2) are two such factors involved in the development of colon cancer, and inhibition of these processes could be important targets for chemoprevention. We have previously shown COX-2 inhibitory activity locally in the colon; e.g. in human fecal water from a group of vegetarians. In this study we focus on 2-pentanone, a frequently occurring compound in common foods such as banana and carrot. The aim was to study the inhibitory effects on prostaglandin production and COX-2 protein expression in tumour necrosis factor-alpha stimulated colon cancer cells (HT29) by radioimmunoassay and Western blotting. 2-Pentanone inhibited both prostaglandin production and COX-2 protein expression in human colon cancer cells. A concentration of 400 mumol/l 2-pentanone inhibited the prostaglandin production by 56.9+/-12.9% which is in the same range as the reference compound NS398 (59.8+/-7.6%). The two highest concentrations of 2-pentanone were further analyzed by Western blot, and 400 micromol/l and 200 micromol/l 2-pentanone resulted in a 53.3+/-9.6% and +/-27.1% reduction of the COX-2 protein levels respectively. Further studies on flavouring compounds, for example 2-pentanone, as colon cancer chemopreventives would be very valuable, and such results may contribute to future dietary recommendations.
Nyckelord
- cyclooxygenase-2 inhibitor
- prostaglandin E-2
- 2-pentanone
- inflammation
- colon cancer cell
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas