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Blocking peptides d...
Blocking peptides decrease tissue transglutaminase processing of gliadin in vitro
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- Hoffmann, Karolina, 1980 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Alminger, Marie, 1957 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Andlid, Thomas, 1963 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Chen, Tingsu (författare)
- Göteborgs universitet,University of Gothenburg
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- Olsson, Olof, 1950 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för växt- och miljövetenskaper,Department of Plant and Environmental Sciences,University of Gothenburg
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- Sandberg, Ann-Sofie, 1951 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- American Chemical Society (ACS), 2009
- 2009
- Engelska.
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Ingår i: Journal of Agricultural and Food Chemistry. - : American Chemical Society (ACS). - 0021-8561 .- 1520-5118. ; 57:21, s. 10150-10155
- Relaterad länk:
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Tissue transglutaminase (tTG) plays an important role in celiac disease pathology as it catalyzes deamidation and cross-linking of specific gluten peptides and converts them into potent epitopes recognized by intestinal T-cells. We investigated whether synthetic peptides with high affinity to gliadin could alter tTG activity on gliadin and whole gluten digest. The immobilized substrates were incubated with synthetic peptides identified by the phage display technique and a control peptide with no affinity to gliadin. Transglutaminase activity was measured with time resolved fluorescence. The mean tTG activity, compared to that of the control without the peptides, was reduced by 31, 33, and 36% for three selected gliadin-binding peptides, and 30% for the peptide pool (P <= 0.001-0.004) when gliadin was the substrate. Finally, substrate specificity experiments suggested that avenin was processed in a manner similar that used for gliadin during in vitro assays with tTG. The results showed that the blocking peptides efficiently reduced tTG processing of gliadin in vitro, and this strategy will be further investigated as an alternative therapy for celiac disease.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- celiac disease
- gliadin
- synthetic peptides
- time resolved fluoroscence
- tissue transglutaminase
- gliadin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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