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A large scale metho...
A large scale method for preparation of plant thylakoids for use in body weight regulation.
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Emek, Sinan C (författare)
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Szilagyi, Anna (författare)
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Akerlund, Hans-Erik (författare)
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visa fler...
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- Albertsson, Per-Åke (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Köhnke, Rickard (författare)
- Lund University,Lunds universitet,Aptitkontroll,Forskargrupper vid Lunds universitet,Appetite Regulation,Lund University Research Groups
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- Holm, Anders (författare)
- Lund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups
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- Erlanson-Albertsson, Charlotte (författare)
- Lund University,Lunds universitet,Aptitkontroll,Forskargrupper vid Lunds universitet,Appetite Regulation,Lund University Research Groups
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visa färre...
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(creator_code:org_t)
- Informa UK Limited, 2010
- 2010
- Engelska.
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Ingår i: Preparative biochemistry & biotechnology. - : Informa UK Limited. - 1532-2297 .- 1082-6068. ; 40:1, s. 13-27
- Relaterad länk:
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http://www.ncbi.nlm....
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- A method for preparation of thylakoids from plant leaves on a large scale is described. The method involves: 1) disruption of the cells with a blender followed by filtration to remove large cell debris and non disrupted cells. 2) precipitation of the thylakoids by adjusting the pH to the isoelectric point, pH 4.7. 3) a washing step by dilution of the precipitate in water followed by precipitation at the same pH. 4) concentration of the precipitate by freeze- thawing or freeze -drying to get the final product. The product is characterized, with respect to protein composition, by SDS-PAGE and mass-spectroscopy, the content of carotenoids, particularly the xanthophylls violaxanthin, antheraxanthin, and zeaxanthin. The thylakoid preparation has about the same capacity to inhibit pancreatic lipase/colipase activity as thylakoids prepared by standard laboratory methods using sucrose in the medium and centrifugation. In a study with mice, it was found that, when the thylakoids were added to the food over 32 days, they significantly reduced the body weight gain and the percentage body fat. The large scale method described here allows studies on the effect of thylakoids in appetite regulation on experimental animals in a longer lasting time and also on humans.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Näringslära (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Nutrition and Dietetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- Adiposity
- drug effects
- Animals
- Cell Fractionation
- methods
- Eating
- drug effects
- Female
- Fractional Precipitation
- Lipase
- metabolism
- Lipid Metabolism
- Mice
- Phytotherapy
- Plant Extracts
- therapeutic use
- Plant Leaves
- chemistry
- metabolism
- Spinacia oleracea
- chemistry
- metabolism
- Thylakoids
- chemistry
- metabolism
- ultrastructure
- Weight Gain
- drug effects
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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