Sökning: onr:"swepub:oai:gup.ub.gu.se/133963" > Two-photon fluoresc...
Fältnamn | Indikatorer | Metadata |
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000 | 02652naa a2200289 4500 | |
001 | oai:gup.ub.gu.se/133963 | |
003 | SwePub | |
008 | 240528s2010 | |||||||||||000 ||eng| | |
024 | 7 | a https://gup.ub.gu.se/publication/1339632 URI |
040 | a (SwePub)gu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Guldbrand, Stina,d 1970u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xgulst |
245 | 1 0 | a Two-photon fluorescence correlation microscopy combined with measurements of point spread function; investigations made in human skin |
264 | 1 | c 2010 |
520 | a Two-photon excitation fluorescence correlation spectroscopy (TPFCS) has been applied in connection to measurements of the point spread function (PSF) for quantitative analysis of sulphorhodamine B (SRB) in excised human skin. The PSF was measured using subresolution fluorescent beads embedded in the skin specimen. The PSF, measured as full width at half maximum (FWHM) was found to be 0.41 ± 0.05 μm in the lateral direction, and 1.2 ± 0.4 μm in the axial direction. The molecular diffusion of SRB inside the skin ranged between 0.5 and 15.0 × 10 −8 cm2/s. The diffusion coefficient is not dependent on depths down to 40 μm. The fluorophores were found to accumulate on the upper layers of the skin. This work is the first TPFCS study in human skin. The results show that TPFCS can be used for quantitative analyses of fluorescent compounds in human skin. | |
650 | 7 | a NATURVETENSKAPx Fysikx Atom- och molekylfysik och optik0 (SwePub)103022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Physical Sciencesx Atom and Molecular Physics and Optics0 (SwePub)103022 hsv//eng |
700 | 1 | a Simonsson, Carl,d 1976u Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry4 aut0 (Swepub:gu)xsimca |
700 | 1 | a Goksör, Mattias,d 1975u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xgokma |
700 | 1 | a Smedh, Maria,d 1968u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xsmema |
700 | 1 | a Ericson, Marica B,d 1974u Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)4 aut0 (Swepub:gu)xemarq |
710 | 2 | a Göteborgs universitetb Institutionen för fysik (GU)4 org |
773 | 0 | t Optics Expressg 18:15, s. 15289-15302q 18:15<15289-15302x 1094-4087 |
856 | 4 8 | u https://gup.ub.gu.se/publication/133963 |
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