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Lack of antibody pr...
Lack of antibody production against Hanganutziu-Deicher (H-D) antigens with N-glycolylneuraminic acid in patients with porcine exposure history.
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Kobayashi, T (författare)
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Yokoyama, I (författare)
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Suzuki, A (författare)
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Abe, M (författare)
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Hayashi, S (författare)
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Matsuda, H (författare)
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Morozumi, K (författare)
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- Breimer, Michael, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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- Rydberg, Lennart, 1944 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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- Groth, C G (författare)
- Karolinska Institutet
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- Tibell, A (författare)
- Karolinska Institutet
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Korsgren, O (författare)
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Takagi, H (författare)
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Nakao, A (författare)
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(creator_code:org_t)
- Wiley, 2000
- 2000
- Engelska.
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Ingår i: Xenotransplantation. - : Wiley. - 0908-665X. ; 7:3, s. 177-80
- Relaterad länk:
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https://gup.ub.gu.se...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The significance of non-alphagalactosyl antigens remains unclear in pig-to-primate xenotransplantation. Hanganutziu-Deicher (H-D) antigens with terminal N-glycolylneuraminic acid (NeuGc) are widely expressed on endothelial cells of mammalian species, with the exception of humans. As baboons and monkeys also express H-D antigens, a pig-to-non-human primate experimental model cannot resolve the question of whether H-D antigens can elicit a potent humoral response in human recipients. The purpose of this study was to elucidate the clinical significance of H-D antigens by examining the sera from patients who have been previously exposed to porcine tissue. After the digestion of porcine aortic endothelial cells (PAEC) by neuraminidase, NeuGc and N-acetylneuraminic acid (NeuAc) were quantitated by HPLC. IgG and IgM antibody levels against H-D antigens were measured by NeuGc-GM3-coated ELISA plates in the sera of patients who had undergone ex vivo kidney perfusion 1 to 3 weeks and 2 years previously (n=2) or had been injected with fetal porcine islets 2 months previously (n= 10). HPLC determined that 9.7x 10(7) NeuAc and 6.3x 10(7) NeuGc residues per cell were released from PAEC by neuraminidase, while 25.7x 10(7) NeuAc and an undetectable level of NeuGc were released from human aortic endothelial cells (HAEC). No significant elevation of IgG or IgM antibody levels against NeuGc-GM3 was observed in sera from patients with a history of porcine exposure. Considering the active production of antibody against the foreign galactosyl antigens after pig-to-human xenotransplantation, some production of antibodies against the equally foreign H-D antigens would be expected, because large amounts of NeuGc terminated saccharides are present in the pig endothelial cell surface. However, no production of antibodies directed to H-D antigens could be found in patients exposed to porcine tissue. Further studies are warranted to explain why H-D antigens do not elicit a significant antibody production.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- Animals
- Antibodies
- Heterophile
- blood
- Antibody Formation
- Antigens
- Heterophile
- immunology
- Aorta
- Cells
- Cultured
- Endothelium
- Vascular
- immunology
- Enzyme-Linked Immunosorbent Assay
- Haplorhini
- Humans
- Immunoglobulin G
- blood
- Immunoglobulin M
- blood
- Neuraminic Acids
- immunology
- Papio
- Swine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Kobayashi, T
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Yokoyama, I
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Suzuki, A
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Abe, M
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Hayashi, S
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Matsuda, H
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visa fler...
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Morozumi, K
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Breimer, Michael ...
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Rydberg, Lennart ...
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Groth, C G
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Tibell, A
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Korsgren, O
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Takagi, H
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Nakao, A
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Kirurgi
- Artiklar i publikationen
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Xenotransplantat ...
- Av lärosätet
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Göteborgs universitet
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Karolinska Institutet