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Identification of p...
Identification of proteins highly expressed in uterine fluid from mice with hydrometra
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- Antonson, P. (författare)
- Karolinska Institutet
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- Nalvarte, I. (författare)
- Karolinska Institutet
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- Varshney, M. (författare)
- Karolinska Institutet
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Xu, L. (författare)
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- Windahl, Sara H, 1971 (författare)
- Gothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research
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Humire, P. (författare)
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- Ohlsson, Claes, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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- Gustafsson, J. A. (författare)
- Karolinska Institutet
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- Dahlman-Wright, K. (författare)
- Karolinska Institutet
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- Engelska.
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Ingår i: Biochemical and Biophysical Research Communications. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 466:4, s. 650-655
- Relaterad länk:
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Estrogen receptor alpha (ER alpha) is an important regulator of the estrous cycle and mice with global ER alpha deletion, as well as some conditional knockout mouse lines, have an interruption in the estrous cycle. In this study we observed that conditional ERa knockout mice where the Cre gene is regulated by the rat insulin promoter (RIP), RIP-Cre/ER alpha(KO) mice, have a 3.7-fold increase in serum 17 beta-estradiol levels, blocked estrous cycle, and develop a fluid-filled uterus (hydrometra). Using a proteomics approach, we identified three proteins, lactoferrin, complement C3 and chitinase 3-like protein 1 (CHI3L1), as highly expressed proteins in hydrometra fluid. The mRNA levels of the corresponding genes were more than 50-fold higher in RIP-Cre/ER alpha(KO) uterus compared to controls. High expression of CHI3L1 in the uterine fluid was not reflected as elevated levels in the serum. The high expression of lactoferrin, complement C3 and CHI3L1 in the uterine fluid, in association with elevated estrogen levels, prompted us to address if the expression of these genes is related to reproduction. However, gonadotropin treatment of mice reduced the uterine expression of these genes in a model of in vitro fertilization. Our findings identify lactoferrin, complement C3 and CHI3L1 as highly expressed proteins in hydrometra fluid in association with chronically elevated serum estradiol levels. (C) 2015 Elsevier Inc. All rights reserved.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- Estrogen receptor
- Estrogen
- Uterus
- Proteomics
- Knockout
- ESTROGEN-RECEPTOR-ALPHA
- HUMAN AMNIOTIC-FLUID
- ER-ALPHA
- PROTEOMICS
- ANALYSIS
- BETA-CELLS
- SUBSTRATE-2
- LACTOFERRIN
- GENERATION
- MECHANISM
- SECRETOME
- Biochemistry & Molecular Biology
- Biophysics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Antonson, P.
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Nalvarte, I.
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Varshney, M.
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Xu, L.
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Windahl, Sara H, ...
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Humire, P.
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visa fler...
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Ohlsson, Claes, ...
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Gustafsson, J. A ...
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Dahlman-Wright, ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Endokrinologi oc ...
- Artiklar i publikationen
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Biochemical and ...
- Av lärosätet
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Göteborgs universitet
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Karolinska Institutet