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Expression, sorting...
Expression, sorting, and segregation of Golgi proteins during germ cell differentiation in the testis
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Au, C. E. (författare)
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Hermo, L. (författare)
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Byrne, E. (författare)
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visa fler...
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Smirle, J. (författare)
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Fazel, A. (författare)
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Simon, P. H. G. (författare)
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Kearney, R. E. (författare)
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Cameron, P. H. (författare)
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Smith, C. E. (författare)
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Vali, H. (författare)
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- Fernandez-Rodriguez, Julia, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Core Facilities, Centre for Cellular Imaging,Core Facilities, Centre for Cellular Imaging
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Ma, K. W. (författare)
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Nilsson, T. (författare)
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Bergeron, J. J. M. (författare)
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(creator_code:org_t)
- 2015
- 2015
- Engelska.
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Ingår i: Molecular Biology of the Cell. - 1059-1524. ; 26:22, s. 4015-4032
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The molecular basis of changes in structure, cellular location, and function of the Golgi apparatus during male germ cell differentiation is unknown. To deduce cognate Golgi proteins, we isolated germ cell Golgi fractions, and 1318 proteins were characterized, with 20 localized in situ. The most abundant protein, GL54D of unknown function, is characterized as a germ cell-specific Golgi-localized type II integral membrane glycoprotein. TM9SF3, also of unknown function, was revealed to be a universal Golgi marker for both somatic and germ cells. During acrosome formation, several Golgi proteins (GBF1, GPP34, GRASP55) localize to both the acrosome and Golgi, while GL54D, TM9SF3, and the Golgi trafficking protein TMED7/p27 are segregated from the acrosome. After acrosome formation, GL54D, TM9SF3, TMED4/p25, and TMED7/p27 continue to mark Golgi identity as it migrates away from the acrosome, while the others (GBF1, GPP34, GRASP55) remain in the acrosome and are progressively lost in later steps of differentiation. Cytoplasmic HSP70.2 and the endoplasmic reticulum luminal protein-folding enzyme PDILT are also Golgi recruited but only during acrosome formation. This resource identifies abundant Golgi proteins that are expressed differentially during mitosis, meiosis, and postacrosome Golgi migration, including the last step of differentiation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- MANNOSE 6-PHOSPHATE RECEPTOR
- CLATHRIN-COATED VESICLES
- IN-VIVO ROLE
- ENDOPLASMIC-RETICULUM
- PLASMA-MEMBRANE
- N-GLYCANS
- 3-DIMENSIONAL
- ARCHITECTURE
- MAMMALIAN SPERMATOGENESIS
- TARGETED DISRUPTION
- CYTOPLASMIC DROPLET
- Cell Biology
- ATES OF AMERICA
- V104
- P8346
- NG XM
- 1982
- AMERICAN JOURNAL OF ANATOMY
- V163
- P283
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Au, C. E.
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Hermo, L.
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Byrne, E.
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Smirle, J.
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Fazel, A.
-
Simon, P. H. G.
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visa fler...
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Kearney, R. E.
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Cameron, P. H.
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Smith, C. E.
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Vali, H.
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Fernandez-Rodrig ...
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Ma, K. W.
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Nilsson, T.
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Bergeron, J. J. ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
- Artiklar i publikationen
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Molecular Biolog ...
- Av lärosätet
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Göteborgs universitet