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Cross-reactivity an...
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Leach, Susannah,1983Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
(författare)
Cross-reactivity and avidity of antibody responses induced in humans by the oral inactivated multivalent enterotoxigenic Escherichia coli (ETEC) vaccine ETVAX
- Artikel/kapitelEngelska2017
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LIBRIS-ID:oai:gup.ub.gu.se/258164
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https://gup.ub.gu.se/publication/258164URI
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https://doi.org/10.1016/j.vaccine.2017.06.006DOI
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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We investigated whether the oral inactivated, multivalent enterotoxigenic Escherichia coli (ETEC) vaccine ETVAX, consisting of four E. coli strains over-expressing the colonisation factors (CFs) CFA/I, CS3, CS5 and CS6, combined with the toxoid LCTBA, could induce cross-reactive antibodies to CFs related to the CFA/I and CS5 families. We also evaluated the avidity of vaccine induced antibodies against the toxoid and CFs. Cross-reactivity was analysed in mucosal (faecal and antibodies in lymphocyte supernatants, ALS) samples, and antibody avidity in serum and ALS samples, from two phase I trials: a primary vaccination study, where two oral doses of ETVAX were given the double mutant heat labile toxin (dmLT) adjuvant at a 2-week interval, and a booster vaccination study, where a single booster dose of ETVAX was given 13-23 months after primary vaccinations. We found that 65-90% of subjects who had responded to CFA/I in ALS or faecal specimens also developed cross-reactive antibodies to the related CFs tested, i.e. CS1, CS14 and CS17, and that approximately 80% of those responding to CS5 also responded to the closely related CS7. For subjects who had developed cross-reactive antibodies, the magnitudes of responses against vaccine CFs and related non-vaccine CFs were comparable. Using both a simple method of antibody avidity determination based on limiting antigen dilution, as well as a chaotropic ELISA method, we found that the avidity of serum and ALS antibodies to key vaccine antigens increased after a late booster dose compared to after primary vaccination. Our results suggest that the cross-reactive antibody responses against multiple CFs may result in expanded ETEC strain coverage of ETVAX and that repeated vaccinations induce vaccine-specific antibodies with increased binding capacity.
Ämnesord och genrebeteckningar
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NATURVETENSKAP Biologi Immunologi hsv//swe
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NATURAL SCIENCES Biological Sciences Immunology hsv//eng
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ETEC vaccine
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Colonisation factors
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Antibodies
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Mucosal IgA
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Cross-reactivity
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Avidity
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factor-antigen-i
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colonization-factor
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immunological memory
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immune-responses
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bangladeshi children
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developing-countries
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diarrheal
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patients
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cell responses
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dmlt adjuvant
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immunogenicity
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Immunology
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Research & Experimental Medicine
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Lundgren, Anna,1974Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xlannj
(författare)
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Carlin, N.
(författare)
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Löfstrand, Madeleine,1986Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xlofma
(författare)
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Svennerholm, Ann-Mari,1947Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xsannx
(författare)
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för mikrobiologi och immunologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Vaccine: Elsevier BV35:32, s. 3966-39730264-410X
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Vaccine
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