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Mass spectrometry i...
Mass spectrometry imaging as a novel approach to measure hippocampal zinc
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- Dowlatshahi Pour, Masoumeh, 1976 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ren, Lin, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Jennische, Eva, 1949 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,University of Gothenburg
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- Lange, Stefan, 1948 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,University of Gothenburg
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- Ewing, Andrew G, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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- Malmberg, Per, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2019
- 2019
- Engelska.
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Ingår i: Journal of Analytical Atomic Spectrometry. - : Royal Society of Chemistry (RSC). - 0267-9477 .- 1364-5544. ; 34:8, s. 1581-1587
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Abstract
Ämnesord
Stäng
- Zinc (Zn2+) is an essential trace element that plays crucial roles in the functioning of hundreds of enzymes and DNA binding transcription factors. Zinc is also an essential neuromodulator and can act as a potent neurotoxin in excitotoxic brain injury after seizures, strokes, and brain trauma where high levels of Zn2+ can cause irreparable brain damage in certain brain regions. However, the mechanism of neurotoxicity has not been fully understood yet and is still under debate. In the present study, we have developed a time of flight secondary ion mass spectrometry (ToF-SIMS) imaging method to investigate the distribution of zinc in the rat brain. The zinc distribution in hippocampus sections from healthy rats and rats exposed to traumatic brain injury was imaged and the results were compared to those from conventional zinc-probe based fluorescence microscopy. Two related zinc species, ZnOH3 + and ZnO2H+, can successfully be visualized by ToF-SIMS in the rat hippocampus. Statistical data analysis of the image data demonstrated a substantial increase of both ZnOH3 + and ZnO2H+ in the zinc related species in the acute brain injury tissue. Our findings positively support the fact that toxic vesicular zinc accumulation might not be the sole source for neuronal degeneration following traumatic brain injuries. Also, we could successfully apply ToF-SIMS imaging for the first time to visualize the zinc content and distribution across hippocampus sections. Consequently, ToF-SIMS is a powerful method to further investigate biological phenomena such as seizures, ischemia, and strokes and also other forms of cellular damage in the central nervous system.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Annan medicinteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Other Medical Engineering (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Nyckelord
- brain
- ion
- copper
- localization
- sections
- tissue
- microscopy
- elements
- injury
- lipids
- Chemistry
- Spectroscopy
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Dowlatshahi Pour ...
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Ren, Lin, 1987
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Jennische, Eva, ...
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Lange, Stefan, 1 ...
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Ewing, Andrew G, ...
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Malmberg, Per, 1 ...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Medicinteknik
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och Annan medicintek ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Neurologi
- Artiklar i publikationen
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Journal of Analy ...
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola