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Nitrogen limitation...
Nitrogen limitation reveals large reserves in metabolic and translational capacities of yeast
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Yu, R. (författare)
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- Campbell, Kate, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Pereira, Rui, 1986 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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visa fler...
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- Björkeroth, Johan, 1990 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Qi, Qi, 1992 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Vorontsov, Egor, 1988 (författare)
- Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics,University of Gothenburg
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- Sihlbom, Carina, 1973 (författare)
- Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics,University of Gothenburg
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- Nielsen, Jens B, 1962 (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Chalmers tekniska högskola,Chalmers University of Technology,BioInnovation Institute (BII)
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- Yu, Tao, 1988 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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visa färre...
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(creator_code:org_t)
- 2020-04-20
- 2020
- Engelska.
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 11:1
- Relaterad länk:
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https://www.nature.c...
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https://research.cha... (primary) (free)
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwisereducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and transcript content. Combining multi-omics analysis with metabolic modeling, we find that seven metabolic superpathways maintain >50% metabolic capacity in reserve, with glucose metabolism maintaining >80% reserve capacity. Cells maintain >50% reserve in translational capacity for 2490 out of 3361 expressed genes (74%), with a disproportionately large reserve dedicated to translating metabolic proteins. Finally, ribosome reserves contain up to 30% sub-stoichiometric ribosomal proteins, with activation of reserve translational capacity associated with selective upregulation of 17 ribosomal proteins. Together, our dataset provides a quantitative link between yeast physiology and cellular economics, which could be leveraged in future cell engineering through targeted proteome streamlining. © 2020, The Author(s).
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Nyckelord
- cell protein
- proteome
- ribosome protein
- transcriptome
- bioengineering
- cell component
- chemostat
- crassulacean acid metabolism
- glucose
- metabolism
- nitrogen
- protein
- proteomics
- yeast
- Article
- biomass production
- cell engineering
- data processing
- fungal cell culture
- gene expression
- glucose metabolism
- high performance liquid chromatography
- liquid chromatography-mass spectrometry
- metabolic capacity
- metabolic flux analysis
- metabolomics
- multiomics
- nitrogen availability
- nitrogen limitation
- nitrogen metabolism
- nonhuman
- nutrient limitation
- protein analysis
- protein expression
- quantitative analysis
- RNA analysis
- RNA extraction
- RNA translation
- Saccharomyces cerevisiae
- steady state
- transcriptome sequencing
- transcriptomics
- upregulation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Yu, R.
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Campbell, Kate, ...
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Pereira, Rui, 19 ...
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Björkeroth, Joha ...
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Qi, Qi, 1992
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Vorontsov, Egor, ...
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visa fler...
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Sihlbom, Carina, ...
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Nielsen, Jens B, ...
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Yu, Tao, 1988
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Cellbiologi
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Annan industriel ...
- Artiklar i publikationen
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Nature Communica ...
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola