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A microsensor-based...
A microsensor-based method for measuring respiration of individual nematodes
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- Maciute, Adele (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Department of marine sciences
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Holovachov, Oleksandr (författare)
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Berg, Peter (författare)
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Glud, Ronnie N. (författare)
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- Broman, Elias (författare)
- Stockholms universitet,Institutionen för ekologi, miljö och botanik,Stockholms universitets Östersjöcentrum
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- Nascimento, Francisco J. A (författare)
- Stockholms universitet,Institutionen för ekologi, miljö och botanik,Stockholms universitets Östersjöcentrum
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- Bonaglia, Stefano, 1983 (författare)
- Stockholms universitet,Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Department of marine sciences,Institutionen för ekologi, miljö och botanik,University of Gothenburg, Sweden; University of Southern Denmark, Denmark
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(creator_code:org_t)
- 2021
- 2021
- Engelska.
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Ingår i: Methods in Ecology and Evolution. - 2041-210X. ; 12:10, s. 1841-1847
- Relaterad länk:
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https://doi.org/10.1...
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https://su.diva-port... (primary) (Raw object)
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Meiofauna (invertebrates that pass through a 1-mm mesh sieve, but are retained on a 40-µm mesh) represent the most abundant and diverse animal group on Earth, but empirical evidence of their role in benthic respiration, production and carbon cycling across ecosystems is not well documented. Moreover, how meiofauna respond to changing oxygen conditions is poorly understood. We further developed an incubation system, in which oxygen and temperature conditions are easily controlled and single meiofaunal nematode respiration is resolved in glass capillary tubes, using Clark-type oxygen microsensor. We performed the respiration measurements after exposing nematodes to different ambient oxygen concentrations, which resulted in 3–60µM O2 during hypoxic and 80–210µM O2 during oxic incubations in close proximity to the respective nematodes. Individual nematode respiration rates ranged from 0.02 to 1.30nmol O2 ind.−1day−1 and were 27% lower during hypoxic than oxic incubations. Rates derived from established allometric relations were on average fourfold higher than our direct measurements. The presented method is suitable for single nematode respiration measurements and can be adapted to a wide range of experimental conditions. Therefore, it can be used to assess meiofauna contribution to ecosystem processes and investigate species-specific responses to changing environmental conditions, for example, oxygen stress, increasing water temperature.
Ämnesord
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Oceanografi, hydrologi och vattenresurser (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Oceanography, Hydrology and Water Resources (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Naturresursteknik -- Annan naturresursteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Environmental Engineering -- Other Environmental Engineering (hsv//eng)
- NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Geokemi (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Geochemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Clark-type microsensor
- hypoxia
- meiobenthos
- meiofauna
- nematodes
- oxygen consumption
- respiration
- Clark-type microsensor
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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