Breast Milk Oligosaccharides Contain Immunomodulatory Glucuronic Acid and LacdiNAc

Jin, ChunshengGothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics (författare)

Breast milk is abundant with functionalized milk oligosaccharides (MOs) to nourish and protect the neonate. Yet we lack a comprehensive understanding of the repertoire and evolution of MOs across Mammalia. We report similar to 400 MO -species associations (>100 novel structures) from milk glycomics of nine mostly understudied species: alpaca, beluga whale, black rhinoceros, bottlenose dolphin, impala, L'Hoest's monkey, pygmy hippopotamus, domestic sheep, and striped dolphin. This revealed the hitherto unknown existence of the LacdiNAc motif (GalNAc beta 1-4GlcNAc) in MOs of all species except alpaca, sheep, and striped dolphin, indicating the widespread occurrence of this potentially antimicrobial motif in MOs. We also characterize glucuronic acid-containing MOs in the milk of impala, dolphins, sheep, and rhinoceros, previously only reported in cows. We demonstrate that these GlcA-MOs exhibit potent immunomodulatory effects. Our study extends the number of known MOs by >15%. Combined with >1900 curated MO -species associations, we characterize MO motif distributions, presenting an exhaustive overview of MO biodiversity.

Lundstrøm, JonGothenburg University,Göteborgs universitet,Wallenberg Centre for Molecular and Translational Medicine,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xljonx (författare)
Korhonen, Emma,1997Gothenburg University,Göteborgs universitet,Wallenberg Centre for Molecular and Translational Medicine,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xkorem (författare)
Luis, Ana S.Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xdejan (författare)
Bojar, DanielGothenburg University,Göteborgs universitet,Wallenberg Centre for Molecular and Translational Medicine,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xbojda (författare)
Göteborgs universitetCore Facilities, Proteomics (creator_code:org_t)

Av författaren/redakt...: Jin, Chunsheng; Lundstrøm, Jon; Korhonen, Emma, ...; Luis, Ana S.; Bojar, Daniel

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