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Thiophene-Based Lig...
Thiophene-Based Ligands for Specific Assignment of Distinct Aβ Pathologies in Alzheimer's Disease
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- Klingstedt, Therése (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Lantz, Linda (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Shirani, Hamid (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Ge, Junyue, 1993 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,Univ Gothenburg, Sweden
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- Hanrieder, Jörg, 1980 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,Univ Gothenburg, Sweden; UCL, England
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- Vidal, Ruben (författare)
- Indiana Univ Sch Med, IN 46202 USA
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- Ghetti, Bernardino (författare)
- Indiana Univ Sch Med, IN 46202 USA
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- Nilsson, Peter (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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(creator_code:org_t)
- AMER CHEMICAL SOC, 2024
- 2024
- Engelska.
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Ingår i: ACS CHEMICAL NEUROSCIENCE. - : AMER CHEMICAL SOC. - 1948-7193. ; 15:7, s. 1581-1595
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Aggregated species of amyloid-beta (A beta) are one of the pathological hallmarks in Alzheimer's disease (AD), and ligands that selectively target different A beta deposits are of great interest. In this study, fluorescent thiophene-based ligands have been used to illustrate the features of different types of A beta deposits found in AD brain tissue. A dual-staining protocol based on two ligands, HS-276 and LL-1, with different photophysical and binding properties, was developed and applied on brain tissue sections from patients affected by sporadic AD or familial AD associated with the PSEN1 A431E mutation. When binding to A beta deposits, the ligands could easily be distinguished for their different fluorescence, and distinct staining patterns were revealed for these two types of AD. In sporadic AD, HS-276 consistently labeled all immunopositive A beta plaques, whereas LL-1 mainly stained cored and neuritic A beta deposits. In the PSEN1 A431E cases, each ligand was binding to specific types of A beta plaques. The ligand-labeled A beta deposits were localized in distinct cortical layers, and a laminar staining pattern could be seen. Biochemical characterization of the A beta aggregates in the individual layers also showed that the variation of ligand binding properties was associated with certain A beta peptide signatures. For the PSEN1 A431E cases, it was concluded that LL-1 was binding to cotton wool plaques, whereas HS-276 mainly stained diffuse A beta deposits. Overall, our findings showed that a combination of ligands was essential to identify distinct aggregated A beta species associated with different forms of AD.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Alzheimer'sdisease
- amyloid-beta
- protein aggregates
- ligands
- fluorescence
- imaging mass spectrometry
- Alzheimer'sdisease; amyloid-beta; protein aggregates; ligands; fluorescence; imaging mass spectrometry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Klingstedt, Ther ...
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Lantz, Linda
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Shirani, Hamid
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Ge, Junyue, 1993
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Hanrieder, Jörg, ...
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Vidal, Ruben
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visa fler...
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Ghetti, Bernardi ...
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Nilsson, Peter
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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ACS CHEMICAL NEU ...
- Av lärosätet
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Göteborgs universitet
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Linköpings universitet