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Demonstration of P2...
Demonstration of P2Y4 purinergic receptors in the HT-29 human colon cancer cell line
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- Delbro, Dick, 1950 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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Nylund, G. (författare)
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- Nordgren, Svante, 1945 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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(creator_code:org_t)
- 2005
- 2005
- Engelska.
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Ingår i: Autonomic & autacoid pharmacology. - 1474-8665. ; 25:4, s. 163-6
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- 1 The aim of the current study was to investigate the existence of P 2 Y(4) purinergic receptors in the HT-29 human colon cancer cell line. 2 We utilized Western blots and immunocytochemistry for the analysis. 3 Western blotting demonstrated two bands that could not be found after the antibody had been preabsorbed with the control peptide, suggesting that both bands are related to the P 2 Y(4) purinergic receptor. 4 Immunocytochemistry showed immunoreactivity for the P 2 Y(4) purinergic receptor localized in the cytoplasm of the HT-29 cells. 5 This is the first demonstration of the protein expression of P 2 Y(4) purinergic receptors in a human colon cancer cell line.
Nyckelord
- Blotting
- Western
- Cytoplasm/metabolism
- HT29 Cells/*metabolism
- Humans
- Immunohistochemistry
- Receptors
- Purinergic P2/*analysis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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