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Localization of Sph...
Localization of Sphingomyelin in Cholesterol Domains by Imaging Mass Spectrometry
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McQuaw, Carolyn M (författare)
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Zheng, Leiliang (författare)
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- Ewing, Andrew G, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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Winograd, Nicholas (författare)
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(creator_code:org_t)
- 2007
- 2007
- Engelska.
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Ingår i: Langmuir. - 0743-7463 .- 1520-5827. ; 23:10, s. 5645-5650
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https://gup.ub.gu.se...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- The location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the necessity of adding fluorescent labels. This system of coexisting immiscible liquid phases shows cholesterol domains with sizes and shapes comparable to those in the fluorescence microscopy literature. The results show that SM localizes with cholesterol and that palmitoyloleoylphosphatidylcholine is excluded. Moreover, the segregation is not complete, and there is a small amount of both phospholipids distributed throughout.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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