Sökning: onr:"swepub:oai:gup.ub.gu.se/99310" >
Tau in cerebrospina...
Tau in cerebrospinal fluid: a sensitive sandwich enzyme-linked immunosorbent assay using tyramide signal amplification.
-
Yamamori, Hidenaga (författare)
-
Khatoon, Sabiha (författare)
-
Grundke-Iqbal, Inge (författare)
-
visa fler...
-
- Blennow, Kaj, 1958 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
-
Ewers, Michael (författare)
-
Hampel, Harald (författare)
-
Iqbal, Khalid (författare)
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
-
Ingår i: Neuroscience letters. - : Elsevier BV. - 0304-3940. ; 418:2, s. 186-9
- Relaterad länk:
-
https://europepmc.or...
-
visa fler...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Tau in cerebrospinal fluid (CSF) has been proposed as a diagnostic marker for Alzheimer's disease (AD). This paper presents a new sensitive sandwich ELISA allowing quantitation of tau from 8 microl CSF/well. A human specific monoclonal tau antibody HT7 was used as a capture antibody and a mixture of polyclonal tau antibodies, 92e and R134d was used as reporter antibodies. Tyramide signal amplification (TSA) technology was used in the last step to increase the sensitivity. With this TSA-ELISA, the lowest detection limit for tau was 14.3 pg/ml. Tau levels in CSF were found to be increased in AD patients (807+/-304 pg/ml, p<0.001) compared with controls (252+/-94 pg/ml). Thirty-five of 38 AD cases (92% sensitivity) yielded signals greater than cutoff, while only 1 of 38 control cases (97% specificity) was greater. A highly significant correlation was found between this assay and a commonly used kit, INNOTEST hTAU Antigen.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Psykiatri (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Psychiatry (hsv//eng)
Nyckelord
- Aged
- Aged
- 80 and over
- Alzheimer Disease
- cerebrospinal fluid
- diagnosis
- Antibodies
- diagnostic use
- Biological Markers
- analysis
- cerebrospinal fluid
- Biotin
- analogs & derivatives
- diagnostic use
- Brain
- metabolism
- physiopathology
- Brain Chemistry
- physiology
- Cerebrospinal Fluid Proteins
- analysis
- chemistry
- metabolism
- Enzyme-Linked Immunosorbent Assay
- methods
- Female
- Humans
- Indicators and Reagents
- Male
- Predictive Value of Tests
- Reproducibility of Results
- Tyramine
- analogs & derivatives
- diagnostic use
- Up-Regulation
- physiology
- tau Proteins
- analysis
- cerebrospinal fluid
- chemistry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas