In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays

Direct electrochemical measurements of glutamate release in vivo were combined with optogenetics in order to examine light-induced control of glutamate neurotransmission in the rodent brain. Self-referenced recordings of glutamate using ceramic-based microelectrode arrays (MEAs) in hippocampus and frontal cortex demonstrated precise optical control of light-induced glutamate release through channelrhodopsin (ChR2) expression in both rat hippocampus and frontal cortex. Although the virus was only injected unilaterally, bilateral and rostro-caudal expression was observed in slice imaging, indicating diffusion and active transport of the viral particles. Methodology for the optogenetic control of glutamate signaling in the rat brain is thoroughly explained with special attention paid to MEA enzyme coating and cleaning for the benefit of other investigators. These data support that optogenetic control of glutamate signaling is robust with certain advantages as compared to other methods to modulate the in vivo control of glutamate signaling.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
Amperometry
Array
Biosensor
Electrochemistry
Glutamate
Glutamate oxidase
Microelectrode
Neurotransmitter
Optogenetics

Pomerleau, FrancoisUniversity of Kentucky Medical Center (författare)
Quintero, Jorge E.University of Kentucky Medical Center (författare)
Huettl, PeterUniversity of Kentucky Medical Center (författare)
Ai, YiUniversity of Kentucky Medical Center (författare)
Jakobsson, JohanLund University,Lunds universitet,Molekylär neurogenetik,Forskargrupper vid Lunds universitet,Molecular Neurogenetics,Lund University Research Groups(Swepub:lu)mphy-jja (författare)
Lundblad, MartinLund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups(Swepub:lu)mphy-mlu (författare)
Heuer, AndreasLund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine(Swepub:lu)med-ah4 (författare)
Slevin, John TUniversity of Kentucky Medical Center (författare)
Gerhardt, Greg A.University of Kentucky Medical Center (författare)
University of Kentucky Medical CenterMolekylär neurogenetik (creator_code:org_t)

Ingår i:NeuromethodsNew York, NY : Springer New York130, s. 327-3511940-60450893-2336

Av författaren/redakt...: Burmeister, Jaso ...; Pomerleau, Franc ...; Quintero, Jorge ...; Huettl, Peter; Ai, Yi; Jakobsson, Johan; visa fler...; Lundblad, Martin; Heuer, Andreas; Slevin, John T; Gerhardt, Greg A ...; visa färre...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Neurovetenskaper

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