Sökning: onr:"swepub:oai:lup.lub.lu.se:1a8a1b82-7531-47a7-962f-485545def431" >
c-Myc antisense oli...
c-Myc antisense oligonucleotides preserve smooth muscle differentiation and reduce negative remodelling following rat carotid arteriotomy
- Artikel/kapitelEngelska2005
Förlag, utgivningsår, omfång ...
-
2005-06-03
-
S. Karger AG,2005
Nummerbeteckningar
-
LIBRIS-ID:oai:lup.lub.lu.se:1a8a1b82-7531-47a7-962f-485545def431
-
https://lup.lub.lu.se/record/238691URI
-
https://doi.org/10.1159/000085379DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:art swepub-publicationtype
-
Ämneskategori:ref swepub-contenttype
Anmärkningar
-
Objectives: The vascular biology of restenosis is complex and not fully understood, thus explaining the lack of effective therapy for its prevention in clinical settings. The role of c-Myc in arteriotomy-induced stenosis, smooth muscle cell (SMC) differentiation and apoptosis was investigated in rat carotids applying full phosphorothioate antisense ( AS) oligonucleotides (ODNs). Methods: Carotid arteries from WKY rats were submitted to arteriotomy and to local application of ODNs through pluronic gel. Apoptosis ( deoxynucleotidyl transferase-mediated dUTP nick end-labelling), SMC differentiation (SM22 immunofluorescence) and vessel morphology and morphometry ( image analysis) were determined 2, 5 and 30 days after injury, respectively. Results: AS ODNs induced a 60% decrease of target c-Myc mRNA 4 h after surgery in comparison to control sense ( S) and scrambled ODN-treated carotids (p < 0.05). A significant 37 and 50% decrease in SM22 protein in the media of S ODN-treated and untreated carotids was detected when compared to uninjured contralateral arteries (p < 0.05). This reduction in SM22 expression was prevented in AS ODN-treated carotids. Stenosis was mainly due to adventitial constrictive remodelling. Lumen area in AS ODN-treated carotids was 35% greater than in control arteries 30 days after surgery (p < 0.05). TUNEL assay revealed increased apoptosis in AS ODN-treated carotids (p < 0.05). Conclusions: c-Myc AS ODNs reduce arteriotomy-induced negative remodelling. This is accompanied by maintained SMC differentiation and greater apoptosis. The combination of reduced c-Myc-induced proliferation and increased apoptosis may thus underlie the less severe remodelling upon treatment with c-Myc mRNA AS ODN.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Galderisi, U
(författare)
-
De Feo, M
(författare)
-
Gomez, MariaLund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups(Swepub:lu)mphy-mgo
(författare)
-
Esposito, S
(författare)
-
Sante, P
(författare)
-
Renzulli, A
(författare)
-
Agozzino, L
(författare)
-
Hellstrand, PerLund University,Lunds universitet,Kärlfysiologi,Forskargrupper vid Lunds universitet,Vascular Physiology,Lund University Research Groups(Swepub:lu)mphy-phe
(författare)
-
Berrino, L
(författare)
-
Cipollaro, M
(författare)
-
Cotrufo, M
(författare)
-
Rossi, F
(författare)
-
Cascino, A
(författare)
-
KärlfysiologiForskargrupper vid Lunds universitet
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Journal of Vascular Research: S. Karger AG42:3, s. 214-2251423-01351018-1172
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Forte, A
-
Galderisi, U
-
De Feo, M
-
Gomez, Maria
-
Esposito, S
-
Sante, P
-
visa fler...
-
Renzulli, A
-
Agozzino, L
-
Hellstrand, Per
-
Berrino, L
-
Cipollaro, M
-
Cotrufo, M
-
Rossi, F
-
Cascino, A
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Kardiologi
- Artiklar i publikationen
-
Journal of Vascu ...
- Av lärosätet
-
Lunds universitet