Abnormal cytoskeletal protein expression in cultured skin fibroblasts form type 1 diabetes mellitus patiens with nephropathy: A proteomic approach

Diabetic nephropathy (DN) develops in about 40% of insulin-dependent type 1 diabetes mellitus (TlDM) patients, and is associated not only with diabetes duration and metabolic control, but also with a genetic predisposition. Constitutive alterations of cytoskeletal proteins may play a role in the development of DN. We investigated the expression of these proteins in cultured skin fibroblasts, obtained from long-term TlDM patients with and without DN but comparable metabolic control, and from matched healthy subjects, by means of 2-DE electrophoresis and MS-MALDI analyses. In T1DM with DN, compared to the other two groups, quantitative analyses revealed an altered expression of 17 spots (p < 0.05-p < 0.01), corresponding to 12 unique proteins. In T1DM with DN, beta-actin and three isoforms of tubulin beta-2 chain, tropomodulin-3, and LASP-1 were decreased, whereas two tubulin beta-4 chain isoforms, one alpha actinin4 isoform, membrane-organizing extension spike protein (MOESIN), FLJ00279 (corresponding to a fragment of myosin heavy chain, non-muscle type A), vinculin, a tropomyosin isoform, and the macrophage capping protein were increased. A shift in caldesmon isoforms was also detected. These results demonstrate an association between DN and the constitutive expression of cytoskeleton proteins in cultured skin fibroblasts from TlDM with DN, which may retain pathophysiologycal implications.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
IDDM PATIENTS
ACTIN POLYMERIZATION
ANTIPORT ACTIVITY
cytoskeletal proteins
PHENOTYPE
ASSIGNMENT
MICROALBUMINURIA
INSULIN
ERM PROTEINS
MESANGIAL CELLS
GENE-EXPRESSION
diabetic nephropathy
fibroblasts

Lori, E. (författare)
Puricelli, L. (författare)
Arrigoni, GiorgioLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)immu-gar (författare)
Vedovato, M. (författare)
Trevisan, R. (författare)
James, PeterLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-pja (författare)
Tiengo, A. (författare)
Tessari, P. (författare)
Institutionen för immunteknologiInstitutioner vid LTH (creator_code:org_t)

Ingår i:Proteomics Clinical Applications: Wiley2:4, s. 492-5031862-83541862-8346

Av författaren/redakt...: Millioni, R.; Lori, E.; Puricelli, L.; Arrigoni, Giorgi ...; Vedovato, M.; Trevisan, R.; visa fler...; James, Peter; Tiengo, A.; Tessari, P.; visa färre...

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