onr:"swepub:oai:lup.lub.lu.se:4583f6a3-7cb0-44b3-b432-cb529b18e9d8"
Sökning: onr:"swepub:oai:lup.lub.lu.se:4583f6a3-7cb0-44b3-b432-cb529b18e9d8" > Screening Method Us...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04000naa a2200337 4500 | |
| 001 | oai:lup.lub.lu.se:4583f6a3-7cb0-44b3-b432-cb529b18e9d8 | |
| 003 | SwePub | |
| 008 | 160404s2012 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://lup.lub.lu.se/record/33475892 URI |
| 024 | 7 | a https://doi.org/10.1021/pr300802g2 DOI |
| 040 | a (SwePub)lu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a art2 swepub-publicationtype |
| 072 | 7 | a ref2 swepub-contenttype |
| 100 | 1 | a Mörtstedt, Harrietu Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)med-hrm |
| 245 | 1 0 | a Screening Method Using Selected Reaction Monitoring for Targeted Proteomics Studies of Nasal Lavage Fluid. |
| 264 | c 2012-12-14 | |
| 264 | 1 | b American Chemical Society (ACS),c 2012 |
| 520 | a Proteomic-based studies of nasal lavage fluid (NLF) may identify molecular pathways associated with disease pathology and new biomarker candidates of upper airway diseases. However, most studies have used rather tedious untargeted MS techniques. Selected reaction monitoring (SRM) is a sensitive and specific technique that can be used with high throughput. In this study, we developed a semiquantitative SRM-based method targeting 244 NLF proteins. The protein set was identified through a literature study in combination with untargeted LC-MS/MS analyses of trypsin-digested NLF samples. The SRM assays were designed using MS/MS data either downloaded from a proteomic data repository or experimentally obtained. Each protein is represented by one to five peptides, resulting in 708 SRM assays. Three to four transitions per assay were used to ensure analyte specificity. The majority (69%) of the assays showed good within-day precision (coefficient of variation ≤20%). The accuracy of the method was evaluated by analyzing four samples prepared with varying amounts of four proteins. Peptide and protein ratios were in good agreement with expected ratios. In conclusion, a high throughput screening method for relative quantification of 244 NLF proteins was developed. The method should be of general use in any proteomic study of the upper airways. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Hälsovetenskapx Arbetsmedicin och miljömedicin0 (SwePub)303032 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Health Sciencesx Occupational Health and Environmental Health0 (SwePub)303032 hsv//eng |
| 700 | 1 | a Kåredal, Monicau Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)ymed-mkr |
| 700 | 1 | a Jönsson, Bo Au Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)ymed-bjo |
| 700 | 1 | a Lindh, Christianu Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)ymed-cli |
| 710 | 2 | a Avdelningen för arbets- och miljömedicinb Institutionen för laboratoriemedicin4 org |
| 773 | 0 | t Journal of Proteome Researchd : American Chemical Society (ACS)x 1535-3893x 1535-3907 |
| 856 | 4 | u http://www.ncbi.nlm.nih.gov/pubmed/23214469?dopt=Abstracty FULLTEXT |
| 856 | 4 | u http://dx.doi.org/10.1021/pr300802gy FULLTEXT |
| 856 | 4 8 | u https://lup.lub.lu.se/record/3347589 |
| 856 | 4 8 | u https://doi.org/10.1021/pr300802g |
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