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Ultrasmall TPGS-PLG...
Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs
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- Wan, Feng (författare)
- University of Copenhagen
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- Bohr, Søren S.R. (författare)
- University of Copenhagen
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- Kłodzińska, Sylvia Natalie (författare)
- University of Copenhagen
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- Jumaa, Haidar (författare)
- University of Copenhagen
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- Huang, Zheng (författare)
- University of Copenhagen
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- Nylander, Tommy (författare)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Thygesen, Mikkel Boas (författare)
- University of Copenhagen
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- Sørensen, Kasper Kildegaard (författare)
- University of Copenhagen
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- Jensen, Knud Jørgen (författare)
- University of Copenhagen
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- Sternberg, Claus (författare)
- Technical University of Denmark
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- Hatzakis, Nikos (författare)
- University of Copenhagen
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- Mørck Nielsen, Hanne (författare)
- University of Copenhagen
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(creator_code:org_t)
- 2019-12-05
- 2020
- Engelska.
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 12:1, s. 380-389
- Relaterad länk:
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http://dx.doi.org/10...
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https://backend.orbi...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Inhaled antibiotic treatment of cystic fibrosis-related bacterial biofilm infections is challenging because of the pathological environment of the lungs. Here, we present an "environment-adaptive" nanoparticle composed of a solid poly lactic-co-glycolic acid (PLGA) core and a mucus-inert, enzymatically cleavable shell of d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) for the site-specific delivery of antibiotics to bacterial biofilms via aerosol administration. The hybrid nanoparticles with ultrasmall size were self-assembled via a nanoprecipitation process by using a facile microfluidic method. The interactions of the nanoparticles with the biological barriers were comprehensively investigated by using cutting-edge techniques (e.g., quartz crystal microbalance with dissipation monitoring, total internal reflection fluorescence microscopy-based particle tracking, in vitro biofilm model cultured in a flow-chamber system, and quantitative imaging analysis). Our results suggest that the mucus-inert, enzymatically cleavable TPGS shell enables the nanoparticles to penetrate through the mucus, accumulate in the deeper layer of the biofilms, and serve as sustained release depot, thereby improving the killing efficacy of azithromycin (a macrolide antibiotic) against biofilm-forming Pseudomonas aeruginosa. In conclusion, the ultrasmall TPGS-PLGA hybrid nanoparticles represent an efficient delivery system to overcome the multiple barriers and release antibiotics in a sustained manner in the vicinity of the biofilm-forming bacteria.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- aerosol administration
- bio-nano interaction
- cystic fibrosis
- Pseudomonas aeruginosa biofilm
- TPGS-PLGA hybrid nanoparticles
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Wan, Feng
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Bohr, Søren S.R.
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Kłodzińska, Sylv ...
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Jumaa, Haidar
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Huang, Zheng
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Nylander, Tommy
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visa fler...
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Thygesen, Mikkel ...
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Sørensen, Kasper ...
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Jensen, Knud Jør ...
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Sternberg, Claus
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Hatzakis, Nikos
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Mørck Nielsen, H ...
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och Biologi
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och Mikrobiologi
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Lunds universitet