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Cell-specific and e...
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Laurie, K. L.
(författare)
Cell-specific and efficient expression in mouse and human B cells by a novel hybrid immunoglobulin promoter in a lentiviral vector
- Artikel/kapitelEngelska2007
Förlag, utgivningsår, omfång ...
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2007-09-13
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Springer Science and Business Media LLC,2007
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:66f4acac-5b09-44a6-ab6f-ee23b64fd185
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https://lup.lub.lu.se/record/968888URI
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https://doi.org/10.1038/sj.gt.3303021DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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The expression of genes specifically in B cells is of great interest in both experimental immunology as well as in future clinical gene therapy. We have constructed a novel enhanced B cell-specific promoter (Igk- E) consisting of an immunoglobulin kappa (Igk) minimal promoter combined with an intronic enhancer sequence and a 30 enhancer sequence from Ig genes. The Igk- E promoter was cloned into a lentiviral vector and used to control expression of enhanced green fluorescent protein (eGFP). Transduction of murine B-cell lymphoma cell lines and activated primary splenic B cells, with IgK-E-eGFP lentivirus, resulted in expression of eGFP, as analysed by flow cytometry, whereas expression in non-B cells was absent. The specificity of the promoter was further examined by transducing Lin bone marrow with Igk-E-eGFP lentivirus and reconstituting lethally irradiated mice. After 16 weeks flow cytometry of lymphoid tissues revealed eGFP expression by CD19(+) cells, but not by CD3(+), CD11b(+), CD11c(+) or Gr-1(+) cells. CD19(+) cells were comprised of both marginal zone B cells and recirculating follicular B cells. Activated human peripheral mononuclear cells were also transduced with Igk-E-eGFP lentivirus under conditions of selective B-cell activation. The Igk-E promoter was able to drive expression of eGFP only in CD19(+) cells, while eGFP was expressed by both spleen focus forming virus and cytomegalovirus constitutive promoters in CD19(+) and CD3(+) lymphocytes. These data demonstrate that in these conditions the Igk-E promoter is cell specific and controls efficient expression of a reporter protein in mouse and human B cells in the context of a lentiviral vector.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Blundell, M. P.
(författare)
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Baxendale, H. E.
(författare)
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Howe, S. J.
(författare)
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Sinclair, J.
(författare)
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Qasim, W.
(författare)
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Brunsberg, U
(författare)
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Thrasher, A. J.
(författare)
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Holmdahl, RikardLund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups(Swepub:lu)infl-rho
(författare)
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Gustafsson, K
(författare)
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ImmunologiForskargrupper vid Lunds universitet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Gene Therapy: Springer Science and Business Media LLC14:23, s. 1623-16310969-71281476-5462
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Till lärosätets databas
- Av författaren/redakt...
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Laurie, K. L.
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Blundell, M. P.
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Baxendale, H. E.
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Howe, S. J.
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Sinclair, J.
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Qasim, W.
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visa fler...
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Brunsberg, U
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Thrasher, A. J.
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Holmdahl, Rikard
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Gustafsson, K
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Immunologi inom ...
- Artiklar i publikationen
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Gene Therapy
- Av lärosätet
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Lunds universitet