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- Rosa, Monica (författare)
DNA pre-condensation with an amino acid-based cationic amphiphile. A viable approach for liposome-based gene delivery
- Artikel/kapitelEngelska2008
Förlag, utgivningsår, omfång ...
- 2009-07-09
- Informa UK Limited,2008
Nummerbeteckningar
- LIBRIS-ID:oai:lup.lub.lu.se:ad364b82-479a-4657-839d-ec945e45edd5
- https://lup.lub.lu.se/record/1199813URI
- https://doi.org/10.1080/09687680701499451DOI
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- Språk:engelska
- Sammanfattning på:engelska
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- A study related to the development and characterization of a new gene delivery system was performed. The approach consists in both the pre-condensation of plasmid DNA with an arginine-based cationic surfactant, arginine-N-lauroyl amide dihydrochloride (ALA), which was found not to be toxic, and the incorporation of the blood protein transferrin (Tf) into the formulations.Two cationic liposome formulations were used, one composed of a mixture of dioleoyl trimethylammoniopropane and cholesterol (DOTAP:Chol) and the other a pH sensitive formulation constituted of DOTAP, Chol, Dioleoyl phosphatidylethanolamine (DOPE) and cholesteryl hemisuccinate (CHEMS).Particles with different ALA/DNA and cationic lipid/DNA charge ratios were produced and a physicochemical characterization of the systems developed was performed. DNA conformational changes in the presence of ALA were studied by Circular Dichroism (CD) and the ALA binding to DNA was followed by surface tension measurements. Insight into the structure and morphology of the various ALA-complexes (complexes composed of ALA, DNA, Tf and liposomes) was obtained by cryogenic-Transmission Electron Microscopy (cryo-TEM) and the sizes of the ALA-complexes were determined through Photon Correlation Spectroscopy (PCS). We found that the transfection capacity of these systems is directly related with the presence of ALA and the lipidic composition. Complexes based on the pH sensitive liposome formulation present better transfection profiles. The correlation between the inner structure, density and size of the ALA-complexes and their biological activity is discussed. Overall, we demonstrate that the presence of ALA improves the transfection efficiency when conjugated with cationic liposome systems.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Kemi Fysikalisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Physical Chemistry hsv//eng
- cationic liposomes
- gene delivery
- amino acid-based amphiphile
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Penacho, Nuno (författare)
- Simoes, Sergio (författare)
- Lima, Maria C P (författare)
- Lindman, BjörnLund University,Lunds universitet,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)fk1-bli (författare)
- Miguel, Maria G (författare)
- Fysikalisk kemiEnheten för fysikalisk och teoretisk kemi (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Molecular Membrane Biology: Informa UK Limited25:1, s. 23-340968-76881464-5203
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- Rosa, Monica
- Penacho, Nuno
- Simoes, Sergio
- Lima, Maria C P
- Lindman, Björn
- Miguel, Maria G
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