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Chondroitin sulfate proteoglycans and microglia prevent migration and integration of grafted Muller stem cells into degenerating retina
- Singhal, Shweta (författare)
- Lawrence, Jean M (författare)
- Bhatia, Bhairavi (författare)
- visa fler...
- Ellis, James S (författare)
- Kwan, Anthony S (författare)
- MacNeil, Angus (författare)
- Luthert, Philip J (författare)
- Fawcett, James W (författare)
- Khaw, Peng T (författare)
- Limb, Astrid (författare)
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- (creator_code:org_t)
- 2008-01-24
- 2008
- Engelska.
- Ingår i: Stem Cells. - : Oxford University Press (OUP). - 1549-4918 .- 1066-5099. ; 26:4, s. 1074-1082
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- At present, there are severe limitations to the successful migration and integration of stem cells transplanted into the degenerated retina to restore visual function. This study investigated the potential role of chondroitin sulfate proteoglycans (CSPGs) and microglia in the migration of human Muller glia with neural stem cell characteristics following subretinal injection into the Lister hooded (LH) and Royal College of Surgeons (RCS) rat retinae. Neonate LH rat retina showed minimal baseline microglial accumulation (CD68-positive cells) that increased significantly 2 weeks after transplantation (p <.001), particularly in the ganglion cell layer (GCL) and inner plexiform layer. In contrast, nontransplanted 5-week-old RCS rat retina showed considerable baseline microglial accumulation in the outer nuclear layer (ONL) and photoreceptor outer segment debris zone (DZ) that further increased (p <.05) throughout the retina 2 weeks after transplantation. Marked deposition of the N-terminal fragment of CSPGs, as well as neurocan and versican, was observed in the DZ of 5-week-old RCS rat retinae, which contrasted with the limited expression of these proteins in the GCL of the adult and neonate LH rat retinae. Staining for CSPGs and CD68 revealed colocalization of these two molecules in cells infiltrating the ONL and DZ of the degenerating RCS rat retina. Enhanced immune suppression with oral prednisolone and intraperitoneal injections of indomethacin caused a reduction in the number of microglia but did not facilitate Muller stem cell migration. However, injection of cells with chondroitinase ABC combined with enhanced immune suppression caused a dramatic increase in the migration of Muller stem cells into all the retinal cell layers. These observations suggest that both microglia and CSPGs constitute a barrier for stem cell migration following transplantation into experimental models of retinal degeneration and that control of matrix deposition and the innate microglial response to neural retina degeneration may need to be addressed when translating cell-based therapies to treat human retinal disease.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- tissue regeneration tissue-specific stem cells
- experimental models
- stem cell transplantation
- adult stem cells
- cell migration
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- Singhal, Shweta
- Lawrence, Jean M
- Bhatia, Bhairavi
- Ellis, James S
- Kwan, Anthony S
- MacNeil, Angus
- visa fler...
- Luthert, Philip ...
- Fawcett, James W
- Perez, Maria The ...
- Khaw, Peng T
- Limb, Astrid
- visa färre...
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