Clonal evolution through genetic bottlenecks and telomere attrition : Potential threats to in vitro data reproducibility

Gisselsson, DavidLund University,Lunds universitet,Cancercellers evolution,Forskargrupper vid Lunds universitet,Pathways of cancer cell evolution,Lund University Research Groups (författare)

Tissue cultures of immortalized human cells, also known as established cell lines, are broadly accessible and cost-efficient tools for biomedical research. We here review potential genetic sources of systematic error in cell line experiments due to clonal evolution in vitro. In particular, the authors highlight alterations in telomere function over prolonged culture and population bottlenecks, respectively, as two commonly overlooked phenomena that can result in significant alterations in cell line genotypes over just one or a few passages in vitro. These alterations may include changes in mutation status of oncogenes and large scale chromosomal imbalances. We introduce a simple list of factors to be avoided in order to reduce the risk of data misinterpretation due to clonal evolution, including unacknowledged in vitro selection pressures, prolonged culture per se, harsh population size reductions, experiments at early phases after establishment, and the employment of cell lines not sufficiently analyzed by high resolution genetic techniques.

Lichtenzstejn, DanielUniversity of Manitoba (författare)
Kachko, PolinaBen Gurion University of the Negev (författare)
Karlsson, JennyLund University,Lunds universitet,Cancercellers evolution,Forskargrupper vid Lunds universitet,Pathways of cancer cell evolution,Lund University Research Groups(Swepub:lu)molm-jka (författare)
Manor, EstherBen Gurion University of the Negev (författare)
Mai, SabineUniversity of Manitoba (författare)
Cancercellers evolutionForskargrupper vid Lunds universitet (creator_code:org_t)

Av författaren/redakt...: Gisselsson, Davi ...; Lichtenzstejn, D ...; Kachko, Polina; Karlsson, Jenny; Manor, Esther; Mai, Sabine

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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