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Activation of human...
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Mata Forsberg, ManuelStockholms universitet,Stockholm University,Institutionen för molekylär biovetenskap, Wenner-Grens institut
(författare)
Activation of human γδ T cells and NK cells by Staphylococcal enterotoxins requires both monocytes and conventional T cells
- Artikel/kapitelEngelska2022
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Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:d6dbd00c-a731-4819-bc8d-ea13d98eb12b
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https://lup.lub.lu.se/record/d6dbd00c-a731-4819-bc8d-ea13d98eb12bURI
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https://doi.org/10.1002/JLB.3A1020-630RRDOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-195723URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Staphylococcal enterotoxins (SE) pose a great threat to human health due to their ability to bypass antigen presentation and activate large amounts of conventional T cells resulting in a cytokine storm potentially leading to toxic shock syndrome. Unconventional T- and NK cells are also activated by SE but the mechanisms remain poorly understood. In this study, the authors aimed to explore the underlying mechanism behind SE-mediated activation of MAIT-, γδ T-, and NK cells in vitro. CBMC or PBMC were stimulated with the toxins SEA, SEH, and TSST-1, and cytokine and cytotoxic responses were analyzed with ELISA and flow cytometry. All toxins induced a broad range of cytokines, perforin and granzyme B, although SEH was not as potent as SEA and TSST-1. SE-induced IFN-γ expression in MAIT-, γδ T-, and NK cells was clearly reduced by neutralization of IL-12, while cytotoxic compounds were not affected at all. Kinetic assays showed that unconventional T cell and NK cell-responses are secondary to the response in conventional T cells. Furthermore, co-cultures of isolated cell populations revealed that the ability of SEA to activate γδ T- and NK cells was fully dependent on the presence of both monocytes and αβ T cells. Lastly, it was found that SE provoked a reduced and delayed cytokine response in infants, particularly within the unconventional T and NK cell populations. This study provides novel insights regarding the activation of unconventional T- and NK cells by SE, which contribute to understanding the vulnerability of young children towards Staphylococcus aureus infections.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Arasa, ClaudiaStockholms universitet,Stockholm University,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)clar1766
(författare)
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van Zwol, WillemienStockholms universitet,Stockholm University,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)wiva6019
(författare)
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Uzunçayir, SibelLund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups(Swepub:lu)si6732uz
(författare)
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Schönbichler, AnnaStockholms universitet,Stockholm University,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)ansc0198
(författare)
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Regenthal, PaulinaLund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups(Swepub:lu)med-pur
(författare)
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Schelin, JennyLund University,Lunds universitet,Teknisk mikrobiologi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Applied Microbiology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)tmb-jse
(författare)
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Lindkvist-Petersson, KarinLund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups(Swepub:lu)mbfy-kpe
(författare)
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Björkander, SophiaStockholms universitet,Stockholm University,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)sobj9073
(författare)
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Sverremark-Ekström, EvaStockholms universitet,Stockholm University,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)esver
(författare)
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Stockholm UniversityInstitutionen för molekylär biovetenskap, Wenner-Grens institut
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Leukocyte Biology111:3, s. 597-6090741-54001938-3673
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