Enzymatic degradation of model cellulose films

Enzymatic degradation of model cellulose films prepared by a spin-coating technique was investigated by ellipsometry. The cellulose films were prior to degradation characterized by ellipsometry, contact angle measurements, ESCA (electron spectroscopy for chemical analysis) and AFM (atomic force microscopy). At enzyme addition to preformed cellulose films an initial adsorption was observed, which was followed by a total interfacial mass decrease due to enzymatic degradation of the cellulose films. The degradation rate was found to be constant during an extended time of hours, whereafter the degradation leveled off. In parallel to the decreased interfacial mass, the cellulose degradation resulted in a thinner and more dilute interfacial film. At long degradation times, however, there was an expansion of the cellulose film. The enzyme concentration affected the degradation rate significantly, with a faster degradation at a higher enzyme concentration. The effects of pH, temperature, ionic strength and stirring rate in the cuvette were also investigated. (c) 2004 Elsevier Inc. All rights reserved.

Malmsten, MartinUppsala universitet,Institutionen för farmaci(Swepub:uu)mamal991 (författare)
Tiberg, FredrikLund University,Lunds universitet,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)fk1-fti (författare)
Callisen, T H (författare)
Damhus, T (författare)
Johansen, K S (författare)
Uppsala universitetInstitutionen för farmaci (creator_code:org_t)

Ingår i:Journal of Colloid and Interface Science: Elsevier BV284:1, s. 99-1061095-71030021-9797

Av författaren/redakt...: Eriksson, Jonny; Malmsten, Martin; Tiberg, Fredrik; Callisen, T H; Damhus, T; Johansen, K S

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