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Purification and ch...
Purification and characterization of a protein binding to the SP6 κ promoter : A potential role for CArG-box binding factor-A in κ transcription
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- Bemark, Mats (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine
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- Olsson, Henric (författare)
- Lund University
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- Heinegård, Dick (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine
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- Leanderson, Tomas (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine
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(creator_code:org_t)
- Elsevier BV, 1998
- 1998
- Engelska.
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Ingår i: Journal of Biological Chemistry. - : Elsevier BV. - 0021-9258. ; 273:30, s. 18881-18890
- Relaterad länk:
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http://dx.doi.org/10... (free)
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http://www.jbc.org/c...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A protein interacting with an A-T-rich region that is a positive control element within the SP6 κ promoter was purified and identified as CArG-box binding factor-A. The purified protein was shown to interact specifically with the coding strand of single-stranded DNA and, with lower affinity, with double-stranded DNA. A mutation that inhibited binding of the protein to the A-T-rich region also aborted the transcriptional stimulatory effect of the region. Two Ets proteins, PU.1 and elf-1, that have previously been shown to bind to an adjacent DNA element were shown to physically interact with CArG- box binding factor.A. An antiserum raised against the protein recognized two different forms indicating either that different splice-forms of CArG-box binding factor-A are expressed, or that the protein is subject to posttranslational modification.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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- art (ämneskategori)
- ref (ämneskategori)
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