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Human herpesvirus-8...
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Szekely, LKarolinska Institutet
(författare)
Human herpesvirus-8-encoded LNA-1 accumulates in heterochromatin- associated nuclear bodies
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
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Microbiology Society,1999
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:1938498
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1938498URI
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https://doi.org/10.1099/0022-1317-80-11-2889DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Subnuclear distribution of the human herpesvirus-8 (HHV-8)- encoded nuclear protein LNA-1 was analysed at high resolution in body cavity (BC) lymphoma-derived cell lines, in cell hybrids between BC cells and various human and mouse cells and in freshly infected K562 and ECV cell lines. Three-dimensional reconstruction of nuclei from optical sections and quantitative analysis of the distribution of LNA-1 fluorescence in relation to chromatin showed that LNA-1 associates preferentially with the border of heterochromatin in the interphase nuclei. This was further confirmed in the following systems: in endo- and exonuclease-digested nuclei, in human–mouse (BC-1–Sp2- 0) hybrids and on chromatin spreads. LNA-1 was found to bind to mitotic chromosomes at random. Epstein–Barr virus (EBV), but not HHV-8, was rapidly lost from mouse–human hybrid cells in parallel with the loss of human chromosomes. HHV-8 could persist on the residual mouse background for more than 8 months. In early human–mouse hybrids that contain a single fused nucleus, LNA-1 preferentially associates with human chromatin. After the gradual loss of the human chromosomes, LNA-1 becomes associated with the murine pericentromeric heterochromatin. In human–human hybrids derived from the fusion of the HHV-8-carrying BCBL-1 cells and the EBV-immortalized lymphoblastoid cell line IB4, LNA-1 did not co-localize with EBNA-1, EBNA-2, EBNA-5 or EBNA-6. LNA-1 was not associated with PML containing ND10 bodies either. DNase but not RNase or detergent treatment of isolated nuclei destroys LNA-1 bodies. In advanced apoptotic cells LNA- 1 bodies remain intact but are not included in the apoptotic bodies themselves.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Kiss, C
(författare)
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Mattsson, K
(författare)
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Kashuba, EKarolinska Institutet
(författare)
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Pokrovskaja, KKarolinska Institutet
(författare)
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Juhasz, A
(författare)
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Holmvall, P
(författare)
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Klein, GKarolinska Institutet
(författare)
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Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:The Journal of general virology: Microbiology Society8080 ( Pt 11), s. 2889-29000022-13171465-2099
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