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Analysis of Na+,K+-...
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Bertorello, AMKarolinska Institutet
(författare)
Analysis of Na+,K+-ATPase motion and incorporation into the plasma membrane in response to G protein-coupled receptor signals in living cells
- Artikel/kapitelEngelska2003
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American Society for Cell Biology (ASCB),2003
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:1945099
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1945099URI
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https://doi.org/10.1091/mbc.e02-06-0367DOI
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Språk:engelska
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Sammanfattning på:engelska
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Dopamine (DA) increases Na+,K+-ATPase activity in lung alveolar epithelial cells. This effect is associated with an increase in Na+,K+-ATPase molecules within the plasma membrane ( Ridge et al., 2002 ). Analysis of Na+,K+-ATPase motion was performed in real-time in alveolar cells stably expressing Na+,K+-ATPase molecules carrying a fluorescent tag (green fluorescent protein) in the α-subunit. The data demonstrate a distinct (random walk) pattern of basal movement of Na+,K+-ATPase–containing vesicles in nontreated cells. DA increased the directional movement (by 3.5 fold) of the vesicles and an increase in their velocity (by 25%) that consequently promoted the incorporation of vesicles into the plasma membrane. The movement of Na+,K+-ATPase–containing vesicles and incorporation into the plasma membrane were microtubule dependent, and disruption of this network perturbed vesicle motion toward the plasma membrane and prevented the increase in the Na+,K+-ATPase activity induced by DA. Thus, recruitment of new Na+,K+-ATPase molecules into the plasma membrane appears to be a major mechanism by which dopamine increases total cell Na+,K+-ATPase activity.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Komarova, Y
(författare)
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Smith, K
(författare)
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Leibiger, IBKarolinska Institutet
(författare)
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Efendiev, R
(författare)
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Pedemonte, CH
(författare)
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Borisy, G
(författare)
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Sznajder, JI
(författare)
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Karolinska Institutet
(creator_code:org_t)
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Ingår i:Molecular biology of the cell: American Society for Cell Biology (ASCB)14:3, s. 1149-11571059-15241939-4586
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