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VEGF-A splice varia...
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Gustafsson, TKarolinska Institutet
(författare)
VEGF-A splice variants and related receptor expression in human skeletal muscle following submaximal exercise
- Artikel/kapitelEngelska2005
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American Physiological Society,2005
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:1950802
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1950802URI
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https://doi.org/10.1152/japplphysiol.01402.2004DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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VEGF-A contributes to muscle tissue angiogenesis following aerobic exercise training. The temporal response of the VEGF-A isoforms and their target receptors has not been comprehensively profiled in human skeletal muscle. We combined submaximal exercise with and without reduced leg blood flow to establish whether ischemia-induced metabolic stress was an important physiological stimuli responsible for regulating the VEGF-A system in humans. Nine healthy men performed two 45-min bouts of one-leg knee-extension exercise, with and without blood flow restriction. Muscle biopsies were obtained at rest and 2 and 6 h after exercise. Expression (mRNA) of the VEGF-A splice variants and related receptors [VEGF receptor (VEGFR)-1, VEGFR-2, and neuropilin-1] was determined by using qPCR. VEGF-Atotal expression increased more robustly after exercise with reduced blood flow, and initially this principally reflected an increase in VEGF-A165. Six hours after exercise, there was a relatively greater increase in VEGF-A189, and this response was not influenced by blood flow conditions. VEGFR-1 mRNA expression increased 2 h after exercise, and neuropilin-1 expression was transiently reduced, while all three receptors increased by 6 h. There was no evidence for the expression of the inhibitory VEGF-A165B variant in human skeletal muscle. Our study, reflecting both VEGF-A ligand and receptors, implicates metabolic perturbation as a regulator of human muscle angiogenesis and demonstrates that VEGF-A splice variants are distinctly regulated. Our findings also indicate that all three receptor genes exhibit different pretranslational regulation, in response to exercise in humans.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ameln, HKarolinska Institutet
(författare)
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Fischer, HKarolinska Institutet
(författare)
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Sundberg, CJKarolinska Institutet
(författare)
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Timmons, JA
(författare)
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Jansson, EKarolinska Institutet
(författare)
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Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of applied physiology (Bethesda, Md. : 1985): American Physiological Society98:6, s. 2137-21468750-75871522-1601
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