Sökning: onr:"swepub:oai:research.chalmers.se:7b4241bc-0dce-41df-9e06-9d9bf11bb0e4" >
Identification of t...
Identification of the cytochrome P450s responsible for the biosynthesis of two types of aporphine alkaloids and their de novo biosynthesis in yeast
-
- Li, Qishuang (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences,China Pharmaceutical University
-
- Jiao, Xiang, 1990 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Li, Xinyi (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
visa fler...
-
- Shi, Wenlong (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Ma, Ying (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Tan, Xiangmei (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Gan, Jingyi (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
Liu, Jimei (författare)
-
- Yang, Jian (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Wang, Jian (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Jin, Baolong (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Chen, Tong (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Su, P. (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Zhao, Yujun (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Zhang, Yifeng (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Tang, Jinfu (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Cui, Guanghong (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Chen, Yun, 1978 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Guo, Juan (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
- Huang, Luqi (författare)
- China Academy of Chinese Medical Sciences (CACMS),China Academy of Chinese Medical Sciences
-
visa färre...
-
(creator_code:org_t)
- 2024
- 2024
- Engelska.
-
Ingår i: Journal of Integrative Plant Biology. - 1672-9072 .- 1744-7909. ; 66:8, s. 1703-1717
- Relaterad länk:
-
https://research.cha...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Aporphine alkaloids have diverse pharmacological activities; however, our understanding of their biosynthesis is relatively limited. Previous studies have classified aporphine alkaloids into two categories based on the configuration and number of substituents of the D-ring and have proposed preliminary biosynthetic pathways for each category. In this study, we identified two specific cytochrome P450 enzymes (CYP80G6 and CYP80Q5) with distinct activities toward (S)-configured and (R)-configured substrates from the herbaceous perennial vine Stephania tetrandra, shedding light on the biosynthetic mechanisms and stereochemical features of these two aporphine alkaloid categories. Additionally, we characterized two CYP719C enzymes (CYP719C3 and CYP719C4) that catalyzed the formation of the methylenedioxy bridge, an essential pharmacophoric group, on the A- and D-rings, respectively, of aporphine alkaloids. Leveraging the functional characterization of these crucial cytochrome P450 enzymes, we reconstructed the biosynthetic pathways for the two types of aporphine alkaloids in budding yeast (Saccharomyces cerevisiae) for the de novo production of compounds such as (R)-glaziovine, (S)-glaziovine, and magnoflorine. This study provides key insight into the biosynthesis of aporphine alkaloids and lays a foundation for producing these valuable compounds through synthetic biology.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- engineered yeast
- CYP719C
- aporphine alkaloids
- biosynthesis
- CYP80
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Li, Qishuang
-
Jiao, Xiang, 199 ...
-
Li, Xinyi
-
Shi, Wenlong
-
Ma, Ying
-
Tan, Xiangmei
-
visa fler...
-
Gan, Jingyi
-
Liu, Jimei
-
Yang, Jian
-
Wang, Jian
-
Jin, Baolong
-
Chen, Tong
-
Su, P.
-
Zhao, Yujun
-
Zhang, Yifeng
-
Tang, Jinfu
-
Cui, Guanghong
-
Chen, Yun, 1978
-
Guo, Juan
-
Huang, Luqi
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Kemi
-
och Organisk kemi
- Artiklar i publikationen
-
Journal of Integ ...
- Av lärosätet
-
Chalmers tekniska högskola